Anti-Cavbeta4 Ca2+ Channel Antibody (N10/7)
Our Anti-Cavbeta4 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N10/7. It detects human, mouse, pig, and rat Cavbeta4 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-054-n10-7-wb-3_{width}x.jpg?v=1626903471)
Immunoblot on adult rat brain membrane (RBM), and adult mouse brain membranes (MBM) from wild-type (WT) and lethargic (lh/lh) mice (left) Lethargic mice courtesy of Dr. Jeff Noebels, Baylor College of Medicine.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-054-n10-7-wb-3_1024x.jpg?v=1626903471)
Product Specific References for Applications and Species
- Confocal Microscopy: Mouse
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Immunoprecipitation: Mouse
- Western Blot: Mouse | Rat | Swine
| Confocal Microscopy: Mouse | ||
| PMID | Dilution | Publication |
| 32616470 | 1:100 | Held, R.G., et al. 2020. Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ channels and Ca2+ Entry. Neuron, . |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 24453333 | 1:500 | Etemad, S., et al. 2014. Differential neuronal targeting of a new and two known calcium channel β4 subunit splice variants correlates with their regulation of gene expression.. Journal of Neuroscience, 1446-1461. |
| 19996312 | 1:250 | Obermair, G.J., et al. 2010. Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons.. Journal of Biological Chemistry, 5776-5791. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 24809506 | 1:1000 | Scamps, F., et al. 2015. Nerve injury induces a Gem-GTPase-dependent downregulation of P/Q-type Ca2+ channels contributing to neurite plasticity in dorsal root ganglion neurons.. Pflügers Archiv: European Journal of Physiology, 351-366. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 23948887 | 1:500 | Daschil, N., et al. 2013. CaV1.2 calcium channel expression in reactive astrocytes is associated with the formation of amyloid-β plaques in an Alzheimer''s disease mouse model.. Journal of Alzheimer''s Disease, 439-451. |
| 22737983 | 1:250 | Ferrándiz-Huertas, C., et al. 2013. Regional expression and subcellular localization of the voltage-gated calcium channel β subunits in the developing mouse brain.. Journal of Neurochemistry, 1095-1107. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 20509142 | 5ug/ml | Ortiz-Miranda, S.I., et al. 2010. Differential modulation of N-type calcium channels by micro-opioid receptors in oxytocinergic versus vasopressinergic neurohypophysial terminals.. Journal of Cellular Physiology, 276-288. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 21228161 | not listed | Chen, J., et al. 2011. Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones. Journal of Neuroscience, 512-525. |
| 19022416 | 15ul | Klemmer, P., et al. 2009. Proteomics analysis of immuno-precipitated synaptic protein complexes. Journal of Proteomics, 82-90. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35176221 | 1:50 | Tan, C., et al. 2022. Rebuilding essential active zone functions within a synapse. Neuron, 1-18. |
| 32176688 | 1:500 | Coste de Bagneaux, P., et al. 2020. A homozygous missense variant in CACNB4 encoding the auxiliary calcium channel beta4 subunit causes a severe neurodevelopmental disorder and impairs channel and non-channel functions. PLoS Genetics, e1008625. |
| 30760886 | 1:1000 | Jin, J., et al. 2020. Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior. Molecular Psychiatry, 1035-1049. |
| 31477143 | 1:1000 | Choi, C.S., et al. 2019. Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels. Molecular Brain, 75. |
| 28874522 | 1:250 | Luo, F., et al. 2017. Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca2+ channels. PNAS: USA, E8081-E8090. |
| 25209271 | 1 : 40 | Liu, C., et al. 2014. The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.. Journal of Neuroscience, 12289-12303. |
| 22737983 | 1:1000 | Ferrándiz-Huertas, C., et al. 2013. Regional expression and subcellular localization of the voltage-gated calcium channel β subunits in the developing mouse brain.. Journal of Neurochemistry, 1095-1107. |
| 21241895 | 1:250 | Kaeser, P.S., et al. 2011. RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction.. Cell, 282-295. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 22187436 | 1:1000 | Li, L., et al. 2012. Up-regulation of Cavβ3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain.. Journal of Biological Chemistry, 6002-6013. |
| 19589863 | 1:500 | Van Gemert, N.G., et al. 2009. Dissociation between rat hippocampal CA1 and dentate gyrus cells in their response to corticosterone: effects on calcium channel protein and current. Endocrinology, 4615-4624. |
| Western Blot: Swine | ||
| PMID | Dilution | Publication |
| 24848355 | 1:1000 | Tharp, D.L., et al. 2014. Ovariectomy increases L-type Ca(2+) channel activity in porcine coronary smooth muscle. Menopause, 661-668. |