Anti-Calnexin (N-terminal region) Antibody (M437)

Name: Anti-Calnexin (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM4371
Price: 360.0 USD
Availability: InStock

1 Citation

Our Calnexin (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Calnexin (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).

Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Calnexin (CM4371), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: CM4371

Volume: 100 µL

Variant

Price:

$360.00

Quantity:

Product Details

Target

Calnexin (N-terminal region)

Target Description

Calnexin is a 90 kDa integral membrane protein located primarily in the endoplasmic reticulum (ER). The structure of calnexin includes a long N-terminal calcium-binding domain that extends into the lumen of the ER and a short, acidic cytosolic domain. Calnexin associates with several cell surface proteins as they pass through the ER, and may be involved in the Ca2+-dependent retention of proteins in the ER. The amino acid sequence of calnexin is highly conserved among various species and is similar in sequence to calreticulin, another Ca2+-binding protein found in the ER. Phosphorylation may regulate the activity of the C-terminal region of Calnexin. Both proline-dependent kinase and casein kinase sites have been identified, and the phosphorylation of these sites may regulate calnexin functions involved with detection of ER protein quality control and transport.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M437

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

CANX

Molecular Weight

Antigen

Clone M437 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human calnexin. This sequence has high homology to similar regions in rat and mouse Calnexin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 90 kDa* protein corresponding to the apparent molecular mass of Calnexin on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Calnexin specifically stains endoplasmic reticulum and related vesicular structures in paraformaldehyde fixed and NP-40 permeabilized cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

IP90, P90

UniProt Details

UniProt (Human): P27824
UniProt (Immunogen Species): P27824

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
28714950	not listed	Wang, TH, et al. 2017. Tid1-S regulates the mitochondrial localization of EGFR in non-small cell lung carcinoma. Oncogenesis, e361.

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Anti-Calnexin (N-terminal region) Antibody (M437)

SKU: CM4371

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Calnexin (N-terminal region) Antibody

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