Name: Anti-Alpha Synuclein (Ser129) Antibody
Brand: PhosphoSolutions
SKU: p1571-129
Price: 415.0 USD
Availability: InStock

Our Anti-Alpha Synuclein (Ser129) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Alpha Synuclein (ser129) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SNCA

Molecular Weight

15 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Alpha Synuclein (Ser129) Antibody

Product Details

Target

Alpha Synuclein (ser129)

Target Description

Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alphasynuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at Ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

SNCA

Molecular Weight

15 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 of rat alpha-aynuclein, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Non-Human Primate

Antibody Registry ID

AB_2492038

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~15 kDa alpha-synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser129

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Alpha synuclein antibody, Alpha-synuclein antibody, Alpha-synuclein, isoform NACP140 antibody, alphaSYN antibody, MGC105443 antibody, MGC110988 antibody, MGC127560 antibody, MGC64356 antibody, NACP antibody, Non A beta component of AD amyloid antibody, Non A4 component of amyloid antibody, Non A4 component of amyloid precursor antibody, Non-A beta component of AD amyloid antibody, Non-A-beta component of alzheimers disease amyloid , precursor of antibody, Non-A4 component of amyloid precursor antibody, Non-A4 component of amyloid, precursor of antibody, OTTHUMP00000218549 antibody, OTTHUMP00000218551 antibody, OTTHUMP00000218552 antibody, OTTHUMP00000218553 antibody, OTTHUMP00000218554 antibody, PARK 1 antibody, PARK 4 antibody, PARK1 antibody, PARK4 antibody, Parkinson disease (autosomal dominant Lewy body) 4 antibody, Parkinson disease familial 1 antibody, SNCA antibody, Snca synuclein alpha (non A4 component of amyloid precursor) antibody, SYN antibody, Synuclein alpha antibody, Synuclein alpha 140 antibody, Synuclein alpha (non A4 component of amyloid precursor) antibody, SYUA_HUMAN antibody

UniProt Details

UniProt (Human): P37840
UniProt (Immunogen Species): P37377

NCBI Gene ID

29219

Product Specific References for Applications and Species

Immunohistochemistry: Rat
Western Blot: Rat

Immunohistochemistry: Rat
PMID	Dilution	Publication
30236122	1:200	Corwin, C., et al. 2018. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. Journal of neuroinflammation, 15(1), p.272.

Western Blot: Rat
PMID	Dilution	Publication
32353461	1:1000	Creed, R.B., et al. 2020. Enhanced susceptibility of PINK1 knockout rats to α-synuclein fibrils. Neuroscience, 437, 64-75.

Product Specific Protocols

Alpha-Synuclein Ser129 IHF Protocol: Download

Western Blot Protocol: Download

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Anti-Alpha Synuclein (Ser129) Antibody

Our Anti-Alpha Synuclein (Ser129) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Alpha Synuclein (ser129) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.