Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Name: Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
Brand: Aves Labs
SKU: ACT-1040
Price: 265.0 USD
Availability: InStock

from Aves Labs Datasheet

filler

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

Clonality

Polyclonal

Applications

Host Species

Chicken

Molecular Weight

42 kDa

Go to Full Product Details

β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 ug total protein, 10 ug total protein, 20 ug total protein); 1' antibody -- ACT (1:1000); 2' antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)

Volume: 400 µL

Variant

Price:

$265

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Product Details

Target

Actin

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

Host Species

Chicken

Molecular Weight

42 kDa

Antigen

Actin purified from Rabbit muscle

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313504

Storage

Store at -20˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze antibodies at -80˚C, unless you want to store them for longer periods of time. Note that each time an antibody preparation is frozen about half its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with Actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB Brain: 1:1000-1:2000

Quality Control

This anti-Actin IgY preparation was analyzed by Western Blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat. # H-1004) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

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UniProt Details

UniProt (Immunogen Species): P63267

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Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody