Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Name: Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody
Brand: Aves Labs
SKU: ACT-1040
Price: 285.0 USD
Availability: InStock

from Aves Labs Datasheet

5 Citations

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Chicken

Format

Mixture of IgY fraction and affinity-purified antibodies

Molecular Weight

42 kDa

Go to Full Product Details

TFPI, TFPI2, and mTFPI were expressed in HeLa or 5637 cells via lentiviral transduction. Expressed exogenous TFPI proteins in cells were confirmed via immunoblot detecting the triple HA tag (cat. ET-HA) fused to their N-termini. Actin (cat. ACT) was used as a loading control. Image from publication CC-BY-4.0. PMID: 36351897

β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 µg total protein, 10 µg total protein, 20 µg total protein); primary antibody -- ACT (1:1000); secondary antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)

Volume: 400 µL

Variant

Price:

$285

Quantity:

Bulk Order Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody

Product Details

Target

Actin

Target Description

Purified actin (42 kDa) isolated from rabbit muscle

Format

Mixture of IgY fraction and affinity-purified antibodies

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

Host Species

Chicken

Molecular Weight

42 kDa

Antigen

Actin purified from rabbit muscle

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313504

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified actin from rabbit muscle emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using actin conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB Brain: 1:1000-1:2000

Quality Control

This anti-Actin IgY preparation was analyzed by Western Blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat. # H-1004) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

UniProt Details

UniProt (Immunogen Species): P63267

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
35263584	not listed	Tian, Songhai, S, et al. 2022. Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Cell reports, 110476.
31484065	not listed	Tian, S., et al. 2019. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Reports, 2517-2526.
30481169	not listed	Tian, S., et al. 2018. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. Plos Biology, e2006951.

Western Blot: Mouse
PMID	Dilution	Publication
36351897	1:1000	Tian, S., et al. 2022. Identification Of TFPI As A Receptor Reveals Recombination-Driven Receptor Switching In Clostridioides Difficile Toxin B Variants. Nature Communications, 6786.
34145250	1:2000	Chen, P., et al. 2021. Structural basis for CSPG4 as a receptor for TcdB and a therapeutic target in Clostridioides difficile infection. Nature Communications, 3748.

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Anti-Actin (Nitrocellulose Membrane Loading Control) Antibody