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Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/51 (left) or N23B/49 (right) TC supe.
Adult rat brain membrane immunoblot.Transfected cell immunofluorescence staining N22/21=green, rabbit anti-Shank1=red, DAPI=blue.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Distribution of SHANK3 immuno-positive cells in different regions of the adult mouse cerebral cortex. N, O: High-magnification views of SHANK3-positive cells in the RSPV region (N) and hippocampal region (O) as shown in image M. Green, SHANK3-positive cells (Cat. 75-344, 1:200); blue, cell nuclei. Scale bars, 300 μm (M); 100 μm (N, O). Image from publication, CC-BY-4.0. PMID: 39693031.Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).
NeuroMab Anti-Shank3 Antibody
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