PhosphoSolutions

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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24 in the second lane (-). Phosphospecificity is shown in the first lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).Western blots showing specific labeling of S23/S24 TnI, cat. p2010-2324 (upper) and TnI; cat. 2010-TnI (lower) in protein lysates of mouse cardiomyocytes. Image from publication CC-BY-4.0. PMID: 36424917
PhosphoSolutions Anti-Troponin I (Cardiac) Ser23/24 Antibody
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Western blot of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein phosphorylated at Ser43 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Troponin I (Cardiac) Ser43 Antibody
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Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-TRPM7 (cytoplasmic region) Antibody
$285
Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.
PhosphoSolutions Anti-TRPM7 (Extracellular region) Antibody
$340
Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569) phospho-specific (lanes 3 & 4).
PhosphoSolutions Anti-TRPM7 (Extracellular region) Antibody
$340
Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513), phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497), TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493), phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-TRPM7 (Ser-1493), Phosphospecific Antibody
$340
Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513), phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497), TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493), phospho-specific (lanes 5 & 6).
PhosphoSolutions Anti-TRPM7 (Ser-1513), Phosphospecific Antibody
$340
Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).
PhosphoSolutions Anti-TRPM7 (Ser-1569), Phosphospecific Antibody
$340
Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M571). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-TRPM8 (Extracellular region) Antibody
$370
Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M572). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-TRPM8 (Extracellular region) Antibody
$340
Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with rabbit polyclonal anti-TRPM8 (extracellular region) without peptide blocking (lane 1) or with peptide blocking (lane 2).Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (TP5701). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-TRPM8 (Extracellular region) Antibody
$340
Immunohistochemistry of Formalin-Fixed Paraffin-Embedded (FFPE) prostate cancer cells showing specific staining of TRPM8. Western Blot of transfected COS cells showing specific immunolabeling of TRPM8.
PhosphoSolutions Anti-TRPM8 Antibody
$445
Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of Ca2+/calmodulin dependent kinase II showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19.
PhosphoSolutions Anti-Tryptophan Hydroxylase (Ser19) Antibody
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Western blot of rat brainstem lysate showing specific immunolabeling of the ~55 kDa TPH protein phosphorylated at Ser260 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third lane.
PhosphoSolutions Anti-Tryptophan Hydroxylase (Ser260) Antibody
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Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of cAMP-dependent protein kinase showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser58.
PhosphoSolutions Anti-Tryptophan Hydroxylase (Ser58) Antibody
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Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein.
PhosphoSolutions Anti-Tryptophan Hydroxylase Antibody
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Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine. Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
PhosphoSolutions Anti-Tubulin, beta III Antibody
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Western blot of PC-12 cell lysate incubated in the absence (-) and presence (+) of okadaic acid (OA, 1 µM for 60 min) showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase phosphorylated at Ser31.
PhosphoSolutions Anti-Tyrosine Hydroxylase (Ser31) Antibody
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Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.
PhosphoSolutions Anti-Tyrosine Hydroxylase (Ser40) Antibody
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Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (Cat. 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat. 2027-THSHP, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight. The image was kindly provided by Eugene Dimitrov, Department of Physiology and Biophysics, Rosalind Franklin University.Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
PhosphoSolutions Anti-Tyrosine Hydroxylase Antibody
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein.Immunofluorescence of a section of rat spinal cord labeled with anti-UCHL1 (Cat. 2060-UCHL1, 1:500, red) and anti-Neurofilament H (Cat. 1451-NFH, 1:25,000, green). The large cells are alpha-motorneurons and UCHL1 fills the cytoplasm of their perikarya and dendrites.
PhosphoSolutions Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Antibody
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~24 kDa UCHL1 protein. Immunostaining of E20 rat cortical neuron and glial culture stained with anti-UCHL1 antibody (Cat. 2061-UCHL1, red, 1:500) and rabbit anti-vimentin antibody (green). The blue is DAPI staining nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-vimentin specifically stains intermediate filaments in fibroblasts and glia cells.
PhosphoSolutions Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Antibody
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Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).
PhosphoSolutions Anti-Unphosphorylated N-Cadherin (Tyr-820) Antibody
$340

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