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Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat1 in human A431 cells. The cells were labeled with mouse monoclonal Stat1 (SM2491) antibody (Right), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. Corresponding phase image is shown to the left.
PhosphoSolutions Anti-Stat1 Antibody
$370
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
PhosphoSolutions Anti-Stat3 (N-terminal region) Antibody
$285
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Stat5 (C-terminal region) Antibody
$370
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Stat5 (Tyr-694), Phosphospecific Antibody
$370
Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.
PhosphoSolutions Anti-Survival Motor Neuron (SMN) Antibody
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PhosphoSolutions Anti-Survivin Antibody
$445
Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-Syk (Central region) antibody at 1:250.
PhosphoSolutions Anti-Syk (Central region) Antibody
$285
Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser549 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes). Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser549 (Cat. p1560-549, green, 1:500). Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin (Ser549) Antibody
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Western blot of rat cortical lysate showing specific labeling of the ~78 kDa synapsin protein phosphorylated at Ser62,67 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-Synapsin (Ser62,67) Antibody
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Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synapsin (Cat. 1926-SYNP, 1:1000, red). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I (100 µL) Antibody
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Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-Synapsin I (C-terminal region) antibody at 1:250 (lane 1) or 1:1000 (lane 2).Immunocytochemical labeling of Synapsin I in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Synapsin I (C-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-Synapsin I (C-terminal region) Antibody
$370
Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Synapsin I (Ser603) Antibody
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Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (Cat. p1560-9, 1:500, red). Cells and photo courtesy of QBMCellScience.Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser9 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Synapsin I (Ser9) Antibody
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Western blot of 10ug of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.Immunostaining of cultured mouse caudate neurons showing punctate distribution of synapsin (Cat. 1925-SYNP, 1:1000, green) and MAP (red). Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synapsin I Antibody
$445
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~78 kDa synapsin I doublet protein.
PhosphoSolutions Anti-Synapsin I Antibody
$385
Western blot of rat brain lysate showing specific immunolabeling of the ~ 16 kDa synaptobrevin protein. Western blot showing specific immunolabeling of Synaptobrevin (VAMP, cat. 1933-SYB, 1:1000) in mouse brain, rat brain, and lamprey spinal cord. Image kindly provided by Karina Vargas, University of Pittsburgh.
PhosphoSolutions Anti-Synaptobrevin (VAMP) Antibody
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145 kDa synaptojanin I protein.
PhosphoSolutions Anti-Synaptojanin 1 Antibody
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Western blot of rat hippocampal lysate showing specific labeling of the ~38 kDa Synaptophysin2 protein. Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synaptophysin (Cat. 1950-SNT2, red, 1:100). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.
PhosphoSolutions Anti-Synaptophysin 2 Antibody
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Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Synaptotagmin (Ser309) Antibody
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Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (Cat. p1570-202, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at -20C for 10 minutes. Photo courtesy of Gang Liu.Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
PhosphoSolutions Anti-Synaptotagmin (Thr202) Antibody
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Western blot of rat cortical lysate showing specific labeling of the ~65 kDa synaptotagmin protein.
PhosphoSolutions Anti-Synaptotagmin 1 Antibody
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Western blot of adult mouse brain lysate. The blot was probed with mouse monoclonal anti-Syntaphilin (N-terminal region) antibody at 1:250 (lane 1), 1:1000 (lane 2), or 1:2000 (lane 3).
PhosphoSolutions Anti-Syntaphilin (N-terminal region) Antibody
$285
Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.
PhosphoSolutions Anti-Syntaxin Antibody
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Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody (TM4081), then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
PhosphoSolutions Anti-Talin (Rod domain) Antibody
$285

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