Anti-GFAP Antibody (N206A/8)

Our mouse GFAP monoclonal antibody from NeuroMab is produced in-house from clone N206A/8. It detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
Adult WT (left) and KO (right) mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
Immunoblot versus low speed pellet samples from rat brain (RBLSP) and wild-type (WT), GFAP knockout (KO) and GFAP-R416W mutant knockin (KI) mice and probed with N206A/8 (left), mutant control(middle) and N52A/42 (right). Mutant control courtesy of Michael Brenner, University of Alabama at Birmingham.
Immunostaining of mouse hippocampal CA1 region showing GFAP and IL1B labeling of astrocytes 28 days after pilocarpine induced status epilepticus (SE). DAPI for nucleic acid counterstain (blue). Image from publication CC-BY-4.0. PMID: 35972937
Immunostaining of mouse hippocampal CA1 region showing GFAP and IL1B labeling of astrocytes 28 days after KA-induced status epilepticus (SE). DAPI for nucleic acid counterstain (blue). Image from publication CC-BY-4.0. PMID: 35972937
Immunostaining of mouse hippocampal CA1 region showing Aif1; green (microglia) and Gfap; red (astrocytes) staining 72 hrs, 10 days and 28 days after pilocarpine-induced status epilepticus (SE), SE (SE Pilo), compared to non-SE control animals (Ctr Pilo). DAPI for nucleic acid counterstain (blue). Image from publication CC-BY-4.0. PMID: 35972937
Confocal images showing labelling for DBI in ECL and GFAP+ glial fibers within lamina X of mouse spinal cord. Image from publication CC-BY-4.0. PMID:36336067
Representative image of GFP positive (white, cat. 75-240, 1:500) astrocytes near radioluminescent particles (RLP) injection site in mouse brain. Nuclei is stained with DAPI (blue). Image from publication CC-BY-4.0. PMID:36634053
Western blot showing a band at the molecular weight corresponding to GFAP (Cat. 75-240, 1:1000) in vehicle- or B9-treated sham and injured mice (top panel). The bottom panel shows the total protein loaded on each lane, which was used for normalization. Image from publication CC-BY-4.0. PMID:38302457
TTX treatment (2 μM, O/N) of primary rat hippocampal neuron cultures (DIV23) increases SAP102 phosphorylation, as observable by Western blot with the phospho-specific SAP102 antibody (α-p-SAP102). α-SAP102, and α-GFAP (Cat. 75-240, 1:2000) served as controls. Image from publication CC-BY-4.0. PMID:38582451
Immunohistochemistry for detection of GFAP in rat hippocampus using anti-GFAP antibody clone N206A/8 (Cat. 75-240) and Streptavidin/Biotin-ABC-Peroxidase Staining Kit (Cat. IH-8106). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (AR-6591). After washing, the primary antibody (Cat. 75-240) was incubated with the sample at a dilution of 1:500 at 4C overnight. Then the biotinylated goat anti-mouse secondary antibody was incubated with the tissue sample. Detection was made using Streptavidin/Biotin-ABC-Peroxidase Staining Kit (" target="_blank">Cat. IH-8106).
Immunohistochemistry for detection of GFAP in rat cerebellum using anti-GFAP antibody clone N206A/8 (Cat. 75-240) and Streptavidin/Biotin-ABC-Peroxidase Staining Kit (Cat. IH-8106). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (AR-6591). After washing, the primary antibody (Cat. 75-240) was incubated with the sample at a dilution of 1:500 at 4C overnight. Then the biotinylated goat anti-mouse secondary antibody was incubated with the tissue sample. Detection was made using Streptavidin/Biotin-ABC-Peroxidase Staining Kit (" target="_blank">Cat. IH-8106).
Immunohistochemistry for detection of GFAP in rat cerebellum using anti-GFAP antibody clone N206A/8 (Cat. 75-240) and Streptavidin/Biotin-ABC-Peroxidase Staining Kit (Cat. IH-8106). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (AR-6591). After washing, the primary antibody (Cat. 75-240) was incubated with the sample at a dilution of 1:500 at 4C overnight. Then the biotinylated goat anti-mouse secondary antibody was incubated with the tissue sample. Detection was made using Streptavidin/Biotin-ABC-Peroxidase Staining Kit (" target="_blank">Cat. IH-8106).
Immunohistochemistry for detection of GFAP in rat cerebellum using anti-GFAP antibody clone N206A/8 (Cat. 75-240) and Streptavidin/Biotin-ABC-Peroxidase Staining Kit (Cat. IH-8106). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (AR-6591). After washing, the primary antibody (Cat. 75-240) was incubated with the sample at a dilution of 1:500 at 4C overnight. Then the biotinylated goat anti-mouse secondary antibody was incubated with the tissue sample. Detection was made using Streptavidin/Biotin-ABC-Peroxidase Staining Kit (" target="_blank">Cat. IH-8106).
Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.
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Drosophila, Guinea Pig, Human, Mouse, Non-Human Primate, Polar Bear, Rat
ELISA, ICC, IHC, WB
Mouse
490
550
594
650
Biotin
Carrier-Free

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Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-240

Volume: 100 µL
Price:
$394.00
Ships: 1-2 business days

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