Primary Antibodies

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Western blot analysis of human A431 epithelial cells treated with 100 nM calyculin A for 30 min. (lanes 1, 3, 5, & 7) then the blot was treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with polyclonal anti-ERK2 (a.a. 181-195) (lanes 1 & 2), anti-ERK2 (Thr-188) (lanes 3 & 4), anti-ERK1/2 (Thr-202/Tyr-204) (lanes  5 & 6), or monoclonal anti-ERK1 (C-terminal region) (lanes 7 & 8).Immunocytochemical labeling of ERK2 in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-ERK2 (EP4071) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot analysis of human A431 epithelial cells untreated (lanes 1 & 4) or treated with 100 nM calyculin A for 30 min. (lanes 2 & 5) or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (C-terminal region) (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).Immunocytochemical labeling of ERK1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal ERK1 (C-terminal region) and detected using appropriate secondary antibodies conjugated to Cy3.
Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).Western blot image of various mouse and human HCC cell lines expressing EphA4 (Cat no EM2801, 1:1000). Image from publication CC-BY-4.0. PMID: 38307859
Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).
Western blot image of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then were probed with anti-EGFR (a.a. 961-972) (lanes 1 & 2), anti-EGFR (Ser-967) (lanes 3 & 4), and anti-EGFR (Ser-1142) (lanes 5 & 6).Immunocytochemical labeling in A431 cells untreated or treated with Calyculin A (50 nM) for 15 min. Cells were labeled with anti-EGFR (a.a. 961-972) or anti-EGFR (Ser-1142) antibodies. The specificity of labeling was demonstrated for each antibody by blocking the signals with their respective blocking peptides, EGFR (a.a. 961-972) (EX1875) and phospho-EGFR (Ser-1142) (EX1935).

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