ImmunoChemistry Technologies

301 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 49 - 72 of 301 products

Figure 1. HRP-Maleimide-conjugated to thiolated antibody. Our Conjugation-Ready HRP-Maleimide reagent (HRP-M) makes it easy for scientists to create their own detection conjugates. The maleimide is linked to the HRP, which facilitates a stable bond with sulfhydryl (SH) groups on the target antibody (shown) or other target protein.

Figure 2. Thiolation. Conjugation-Ready HRP Maleimide links to proteins through free SH groups, which often must be added to the protein using Traut’s reagent prior to conjugation.

ImmunoChemistry Technologies Conjugation-Ready HRP Maleimide

$227

ImmunoChemistry Technologies ELISA Plate Sealing Covers

$10.30

Figure 1. Optimization of double-antigen sandwich ELISA. (a) Capture (0.5 to 5.0 µg/mL) and detection antigen (0.2 (green), 0.4 (light blue), 0.8 (yellow), and 1.6 (purple)) were used to optimize capture and detection antigen (N protein). Capture antigen was diluted with Antigen Coating Buffer. From Figure 1a of Cordero-Ortiz, M, et al. (2023) Animals (Basel) 13, 3487 doi: 10.3390/ani13223487. Used under CC License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

ImmunoChemistry Technologies Antigen Coating Buffer, 5X

From $55.90

ImmunoChemistry Technologies Neptune Assay Diluent

From $59

ImmunoChemistry Technologies Costar® 96-Well EIA/RIA Stripwell Plate

$7.50

Figure 1. Propidium iodide excitation and emission spectra.

Figure 2. HL-60 cells were treated with a drug, then stained with FLICA® FAM-VAD-FMK poly-caspase inhibitor reagent (green) (Cat. 92) and propidium iodide (red) (PI). Scanning laser cytometer analysis. Four populations of cells were detected: (A) Unstained live cells, (B) red necrotic cells (PI), (C) green and red late apoptotic cells (FAM-FLICA® plus PI), and (D) green early apoptotic cells (FAM-FLICA®). Data courtesy of Dr. Z. Darzynkiewicz, Brander Cancer Center, NY.

ImmunoChemistry Technologies Propidium Iodide Stain

$23.40

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (Cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

$264

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $212.20

Figure 1. Control Alzheimer's Disease (AD) macrophages and curcumin-treated AD macrophages were exposed to FITC-labeled Aß and stained with SR-DEVD-FMK (Cat. 931/932). Macrophages that engulf FITC-Aß fluoresce green (right). Caspase-positive cells fluoresce red (left). Essentially all of the untreated AD macrophages engulfed FITC-Aß and became apoptotic. Curcumin-treated AD macrophages engulfed FITC-Aß but did not become apoptotic. Data courtesy of Dr. Milan Fiala, UCLA 072205.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-3/7 (DEVD) Assay Kit

From $222.50

ImmunoChemistry Technologies UEA-I-FITC, Conjugated

$200

ImmunoChemistry Technologies UEA-I-Biotin, Conjugated

$200

ImmunoChemistry Technologies PNA-R-PE, Conjugated

$234

ImmunoChemistry Technologies PNA-FITC, Conjugated

$200

ImmunoChemistry Technologies PNA-Biotin, Conjugated

$200

ImmunoChemistry Technologies ConA-FITC, Conjugated

$200

ImmunoChemistry Technologies ConA-Biotin Conjugated

$177

ImmunoChemistry Technologies WGA-FITC, Conjugated

$200

ImmunoChemistry Technologies WGA-Biotin, Conjugated

$200

ImmunoChemistry Technologies Sheep Serum

$70

ImmunoChemistry Technologies Rabbit Serum

$70

ImmunoChemistry Technologies Mouse Serum

$70

ImmunoChemistry Technologies Goat Serum

$70

ImmunoChemistry Technologies Donkey Serum

$70

ImmunoChemistry Technologies Bovine Serum

$70

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