Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects rat NGFR/p75 neurotrophin receptor (p75NTR), and is Protein G purified IgG. It is validated for use in ELISA, IHC-Frozen, WB.
Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG). (A) Detection of p75NTR using mouse monoclonal antibody to rat p75NTR [MC192] M-006-100. (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Using Flow Cytometry, this antibody has frequently been employed for panning to isolate p75NTR-expressing rat cells. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity). Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Protein G purified IgG
Monoclonal
MC192
IgG1
ELISA, IHC, WB
Mouse
NGF receptor
Rat
Rat
Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. The MC192 is supplied in lyophilized form from Protein G-purified hybridoma cell culture supernatants. The lyophilized antibody is stable when stored at 2-8°C or -20°C. After reconstitution undiluted aliquots should be kept at -20°C for up to six months. For additional stability Glycerol (1:1) may be added after reconstitution. Repetitive freeze/thaw cycle should be avoided.
Lyophilized
Lyophilized from a solution containing 0.1% Trehalose in PBS pH 7.4
IHC (lightly fixed), ELISA, WB, Flow Cytometry (2 ug per 10^6 cells) IP (non-reducing conditions only!; do not use reducing agents such as DTT or beta-mercaptoethanol), Traditional formalin fixed paraffin embedded immunohistochemistry is NOT recommended with MC192. Motor neuron isolation, Gene/Toxin Delivery to rat sensory/motor neurons. A working solution of 1-2 µg/mL was determined by immunohistochemical staining on 4% paraformaldehyde fixed, or alcohol fixed rat spinal cord and brain. For non-denatured WB, 1-5 µg/mL was found to be suitable with suitable controls (PC12 lysate). ELISA: detection only, 1-5 µg/mL has been suggested in literature. Immunoprecipitation: 5 µg/mL, > 0.5% triton X-100 buffer/500 ug/lysate; PC12 positive control strong suggested. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984).
Unconjugated
MC192 is specific only for RAT NGFR, no reactivity to Human or mouse NGFR has been reported This monoclonal antibody has been tested for immunohistochemical localisation of p75NTR-expressing rat cells in the spinal cord and brain. This monoclonal antibody does not cross react with p75NTR-expressing cells in other species.
![Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG). (A) Detection of p75NTR using mouse monoclonal antibody to rat p75NTR [MC192] M-006-100. (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.](http://www.antibodiesinc.com/cdn/shop/files/m-006-100-ihc-1_120x120.jpg?v=1759274284)
![Immunohistochemical staining of p75NTR in rat forebrain using mouse monoclonal antibody to rat p75NTR [MC192], catalogue number M-006-50. Paraformaldehyde fixed rat brain sections were incubated with 1 µg/mL antibody overnight, followed by incubation with biotinylated rabbit anti-mouse IgG conjugate. The immunocomplex was then visualised with DAB . Neurons, in the septal nuclei of basal forebrain, known to be cholinergic neurons, show strong staining for p75NTR.](http://www.antibodiesinc.com/cdn/shop/files/m-006-100-ihc-2_120x120.jpg?v=1759274284)
![Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG). (A) Detection of p75NTR using mouse monoclonal antibody to rat p75NTR [MC192] M-006-100. (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.](http://www.antibodiesinc.com/cdn/shop/files/m-006-100-ihc-1_1600x.jpg?v=1759274284)
