Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb (N241A/34)

Name: Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-253-CF
Price: 510.0 USD
Availability: InStock

Our chicken Anti-Dardarin/LRRK2, C-terminus recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N241A/34. It detects drosophila, human, mouse, non-human primate, and rat Dardarin/LRRK2, C-terminus and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb

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Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

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Data Sheet

KO Validated

SKU: 78-253-CF

Volume: 100 µL

Variant

Price:

$510.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Dardarin/LRRK2, C-terminus

Scientific Background

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N241A/34

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 970-2527 (C-terminus) of human LRRK2 (accession number Q5SS00) produced recombinantly in E. Coli.

Immunogen Species

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N241A/34 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N241A/34 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Buffer

PBS

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated, Carrier Free

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt ID

Q5S007

Shipping

Shipped on ice packs

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Anti-LRRK2/Dardarin, C-Terminus Recombinant Chicken Chimeric mAb (N241A/34)

SKU: 78-253-CF

Product Details

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