Fura-2, AM Ester Fluorescent Calcium Indicator

Name: Fura-2, AM Ester Fluorescent Calcium Indicator
Brand: Antibodies Incorporated
SKU: 81-3018
Price: 269.0 USD
Availability: InStock

Our Fura-2 AM Ester is a fluorescent calcium indicator that can be loaded into cells via incubation. Calcium imaging is widely used to measure neuronal activity.

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Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-3018

Size: 10 x 100 µg

Variant

Price:

$269.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Fura-2, AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once it enters cells. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.

Applications

EM, ICC, IHC

Molecular Weight

1001.9 Da

Excitation / Emission

Kd: 145 nM, Ex: 363 nm, Em: 512 nm (no Ca2+); Ex: 335 nm, Em: 505 nm (high Ca2+)

Storage

Store at –20°C, with desiccant; protect from light.

Physical State

Solid

Specificity

Membrane-permeant, ratiometric; can be loaded into cells via incubation.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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Fura-2, AM Ester Fluorescent Calcium Indicator

SKU: 81-3018

Product Details

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