Fluo-3, Pentaammonium Salt Fluorescent Calcium Indicator

Name: Fluo-3, Pentaammonium Salt Fluorescent Calcium Indicator
Brand: Antibodies Incorporated
SKU: 81-3000
Price: 249.0 USD
Availability: InStock

Our Fluo-3, Pentaammonium Salt is a non-ratiometric calcium indicator that increases in fluorescence intensity upon calcium binding. Calcium imaging is widely used to measure neuronal activity.

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Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-3000

Size: 1 mg

Variant

Price:

$249.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fluo-3, pentaammonium is a membrane-impermeant calcium indicator, but can be loaded into cells via microinjection or scrape loading. As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed.

Applications

EM, ICC, IHC

Molecular Weight

854.7 Da

Excitation / Emission

Kd: 450 nM, Ex: 506 nm, Em: 526 nm

Storage

Store at 2°C to 8°C, with desiccant; protect from light.

Physical State

Solid

Specificity

Membrane-impermeant, non-ratiometric; can be loaded into cells via microinjection or scrape loading.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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Fluo-3, Pentaammonium Salt Fluorescent Calcium Indicator

SKU: 81-3000

Product Details

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