Anti-Pancortin Antibody (K96/7)

Name: Anti-Pancortin Antibody
Brand: NeuroMab
SKU: 75-042
Price: 329.0 USD
Availability: InStock

Our Anti-Pancortin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K96/7. It is KO validated, detects human, mouse, and rat Pancortin, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) or N52A/42 (right) TC supe.$

Adult rat whole brain immunohistochemistry.

Immunoblot versus lysates from adult wild-type (+/+) and Pancortin knockout (-/-) mouse cortex probed with K96/7. Data courtesy of Mark Mattson (NIA/NIH) and reproduced with permission from the Journal of Neuroscience (2007, Cheng et al, PMID 17301160).

Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with K96/7 (left) or N52A/42 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-042

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pancortin

Scientific Background

Olfactomedin 1 is encoded by the gene OLFM1. While the exact function of OLFM1 is unknown, its abundant expression in the brain suggests it may have an essential role in nerve tissue. Diseases associated with OLFM1 include Ectopic Pregnancy and Neuroblastoma.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K96/7

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

OLFM1 NOE1 NOEL1

Molecular Weight

55-70 kDa (Pancortin-1 and -3), 15-20 kDa (Pancortin-2 and -4)

Immunogen

Synthetic peptide of amino acids 131-150 of human Pancortin (GLESKFKQVEE SHKQHLARQ, accession number Q99784)

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2267633

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Noelin (Neuronal olfactomedin-related ER localized protein) (Olfactomedin-1)

UniProt ID

Q99784

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse | Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
27787612	1:100	Koch, M.A., et al. 2017. Mutated olfactomedin 1 in the interphotoreceptor matrix of the mouse retina causes functional deficits and vulnerability to light damage. Histochemistry and Cell Biology, 453-469.

Western Blot: Mouse
PMID	Dilution	Publication
27787612	1:1000	Koch, M.A., et al. 2017. Mutated olfactomedin 1 in the interphotoreceptor matrix of the mouse retina causes functional deficits and vulnerability to light damage. Histochemistry and Cell Biology, 453-469.
20595200	1:2000	Bohr, D.C., et al. 2011. Increased expression of olfactomedin-1 and myocilin in podocytes during puromycin aminonucleoside nephrosis.. Nephrology, dialysis, transplantation, 83-92.

Western Blot: Rat
PMID	Dilution	Publication
22992957	1:1000	Rice, H.C., et al. 2012. Pancortins interact with amyloid precursor protein and modulate cortical cell migration.. Development, 3986-3996.
20595200	1:2000	Bohr, D.C., et al. 2011. Increased expression of olfactomedin-1 and myocilin in podocytes during puromycin aminonucleoside nephrosis.. Nephrology, dialysis, transplantation, 83-92.

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Anti-Pancortin Antibody (K96/7)

SKU: 75-042

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Pancortin Antibody

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