AM4-66 Nerve Terminal Dye

Name: AM4-66 Nerve Terminal Dye
Brand: Antibodies Incorporated
SKU: 81-8024
Price: 359.0 USD
Availability: InStock

Our AM4-66 Nerve Terminal Dye is a spectrally identical, fixable analog of SynaptoRed™ C2, a widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions.

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Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-8024

Size: 1 mg

Variant

Price:

$359.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

Nerve terminal dyes have highly hydrophilic, cationically charged head groups at one end with lipophilic tails at the other end. They are virtually non-fluorescent in aqueous solution, but become intensely fluorescent in membranes. Following nerve stimulation, the dye molecules are internalized in newly formed endocytic vesicles. During exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescence signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity. AM4-66 is a fixable red fluorescent nerve terminal probe that is spectrally identical to SynaptoRed™ C2 (also called FM®4-64). but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.

Applications

EM, ICC, IHC

Molecular Weight

872.85 Da

Excitation / Emission

Ex: 510 nm, Em: 750 nm (in membranes)

Storage

Store at 2°C to 8°C, with desiccant; protect from light.

Physical State

Solid

Specificity

For use in real-time imaging of live/intact cells and cells fixed after staining in formaldehyde.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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AM4-66 Nerve Terminal Dye

SKU: 81-8024

Product Details

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