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Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained using Swine Annexin V-Fluorescein Assay (cat. 9142). Annexin V-Fluorescein labeled only 13.1% of control cells (UR + LR = 11.0% + 3.1%, left), whereas staurosporine induced Annexin V-Fluorescein labeling in 96.7% of cells (UR + LR = 32.7% + 64.0%, right). Staurosporine also significantly increased the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 227:19.

ImmunoChemistry Technologies Swine Annexin V-Fluorescein Apoptosis Assay Kit

$469

PhosphoSolutions Talin Phospho-Regulation Antibody Kit

$459

ImmunoChemistry Technologies Tetramethylbenizidine IHC Peroxidase

From $88

ImmunoChemistry Technologies TMB 1-Component HRP Membrane Substrate (SUBM)

$66

ImmunoChemistry Technologies TMB 1-Component HRP Microwell Substrate (SUBT)

From $46.10

ImmunoChemistry Technologies TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)

$58.20

ImmunoChemistry Technologies TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)

From $48.20

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine to induce apoptosis, then stained with TMRE, and photographed using a photomicroscope using a green excitation filter at 510-560 nm and a 570-620 nm emission filter (left). Healthy cells, containing mitochondria with polarized inner membranes fluoresce bright orange (A) compared with apoptotic cells with depolarized mitochondria(B). The corresponding DIC images are shown (right) (Dr. Brian Lee, ICT).

Figure 2. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRE - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$299

Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$299

ImmunoChemistry Technologies Tris Buffer, 10X

From $60

ImmunoChemistry Technologies Tris-HCL Buffer, 10X

From $60

ImmunoChemistry Technologies Triton X100

From $60

PhosphoSolutions TRPM7 Phospho-Regulation Antibody Kit

$510

ImmunoChemistry Technologies Trypsin Reagent

From $70

ImmunoChemistry Technologies Tumor Necrosis Factor alpha

$683.90

ImmunoChemistry Technologies Tumor Necrosis Factor beta

$370.80

ImmunoChemistry Technologies Tween 20

From $60

ImmunoChemistry Technologies UEA-I-Biotin, Conjugated

$200

ImmunoChemistry Technologies UEA-I-FITC, Conjugated

$200

Figure shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.

ImmunoChemistry Technologies Ultra Pure ADP by Cell Technology

From $405

ImmunoChemistry Technologies Universal Antibody Dilution Buffer™

From $60

ImmunoChemistry Technologies Urease Conjugated with Biotin

$211

PhosphoSolutions Vav Phospho-Regulation Antibody Kit

$459

PhosphoSolutions VEGFR Phospho-Regulation Antibody Kit

$459

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