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Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight® 594.
PhosphoSolutions Anti-Sox2 (Thr-118), Phosphospecific Antibody
$340
Western blot of mouse F9 stem cells untreated (lanes 1 & 3) and treated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 antibody (lanes 1 & 2) and rabbit polyclonal Sox2 (Thr-128) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-128) phospho-specific (SP0381). The antibody was detected using goat anti-rabbit Ig:DyLight® 594.
PhosphoSolutions Anti-Sox2 (Thr-128), Phosphospecific Antibody
$340
Immunofluorescent staining of NTERA2 cells stained with Aves Labs anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Aves Labs anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.
Aves Labs Anti-Sox2 Antibody
From $140
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Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.Immunofluoresence of FLAG-tagged SOX2 transfected COS cells showing SOX2 staining in red (cat. 1907-SOX2, 1:20) and FLAG staining in green, causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.
PhosphoSolutions Anti-SOX2 Antibody
From $160
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Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Sox2 (SM5511). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Sox2 Antibody
$285
Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human SK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-SK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).Immunocytochemical labeling of sphingosine kinase 1 (SPHK1) in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-SPHK1 (SM5401). The antibody was detected using goat anti-rat DyLight® 594.
PhosphoSolutions Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein. Blots were probed with rabbit polyclonals anti-SK1 (Central Region) (SP1621) at 1:1000 (lane 1) and 1:4000 (lane 2), and anti-SK1 (C-terminal region) (SP5421) at 1:1000 (lane 3) and 1:4000 (lane 4).The protein expression levels of DGKα, SphK1 (cat. SP5421, 1:1000) and p-SphK1(Ser225) (cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305
PhosphoSolutions Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).
PhosphoSolutions Anti-Sphingosine Kinase 1 (Central region) Antibody
$340
Western blot image of recombinant his-tagged human SK1 protein that was phosphorylated with ERK-2. Blots were probed with anti-SK1 (Central Region) (SP1621; lanes 1-3) and anti-SK1 (Ser-225) (SP1641; lanes 4-6). Both antibodies were used in the presence of no peptide (lanes 1 & 4), phospho-SK1 (Ser-225) peptide (SX1645; lanes 2 & 5), or unphosphorylated SK1 (Ser-225) peptide (SX1625; lanes 3 & 6).Western blot of HeLa stimulated with calyculin A (lanes 1-4). The blots were untreated (lane 1 & 3) or treated with lambda phosphatase (lane 2 & 4), then probed with anti-SK1 (Central region) SP1621 (lanes 1 & 2) or anti-SK1 (Ser-225) SP1641 (lanes 3 & 4).
PhosphoSolutions Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4). The blots were probed with rabbit polyclonal anti-SK2 (N-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-SK2 (N-terminal region) at 1:250 (lane 3) or 1:1000 (lane 4).
PhosphoSolutions Anti-Sphingosine Kinase 2 (N-terminal region) Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Sphingosine Kinase 2 (N-terminal region) Antibody
$340
Western blot of human recombinant SK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-SK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-SK2 (Thr-578) (lanes 3, 4, 7, & 8).
PhosphoSolutions Anti-Sphingosine Kinase 2 (Thr-578), Phosphospecific Antibody
$340
Immunofluorescent staining of mock- or 50 µM chloroquine-treated HeLa cells using 2 µg/mL chicken anti-SQSTM1 (p62) antibody (green). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). Anti-SQSTM1 (p62) specifically stains the autophagosomes in HeLa cells. ​Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.Western blotting of mock- or 50 µM chloroquine-treated HeLa cell lysates with Aves Labs chicken anti-SQSTM1 (p62) antibody at 1:5000 (0.04 µg/ml) and 1:10000 (0.02µg/ml) dilutions and detected with goat anti-chicken HRP. Aves Labs Chicken anti-SQSTM1 (p62) recognizes endogenous SQSTM1 (p62) in HeLa cells at ~62 kDa. Chloroquine treatment increases the levels of SQSTM1 (p62) protein by inhibiting autophagy.
Aves Labs Anti-SQSTM1 Antibody
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NeuroMab Anti-SST/Somatostatin Antibody
$329
NeuroMab Anti-SST/Somatostatin Antibody (BSA and Azide free)
$475
NeuroMab Anti-SST/Somatostatin Antibody FL490 Conjugate
$474
NeuroMab Anti-SST/Somatostatin Antibody FL550 Conjugate
$474
NeuroMab Anti-SST/Somatostatin Antibody FL594 Conjugate
$474
NeuroMab Anti-SST/Somatostatin Antibody FL650 Conjugate
$474
Western blot of rat synaptic plasma membrane (SPM) lysate showing specific immunolabeling of the ~36 kDa stargazin protein.
PhosphoSolutions Anti-Stargazin Antibody
From $160
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Western blot analysis of A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
PhosphoSolutions Anti-Stat1 (Tyr-701), Phosphospecific Antibody
$340
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat1 in human A431 cells. The cells were labeled with mouse monoclonal Stat1 (SM2491) antibody (Right), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. Corresponding phase image is shown to the left.
PhosphoSolutions Anti-Stat1 Antibody
$370
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).
PhosphoSolutions Anti-Stat3 (N-terminal region) Antibody
$285
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-Stat5 (C-terminal region) Antibody
$370

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