Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

Name: Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody
Brand: Aves Labs
SKU: CNP
Price: 470.0 USD
Availability: InStock

8 Citations

This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.

A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.

CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.

A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: CNP

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Murine CNPase (EC 3.1.4.37)

Scientific Background

Murine CNPase (EC 3.1.4.37) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

CNP

Molecular Weight

47 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313538

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:500-1:1000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 3.1.4.37

UniProt ID

P09543

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Rat
Western Blot: Mouse| Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
29251594	1:300	Lin, J.P., et al. 2017. LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair. eLife, e30498.

Immunocytochemistry: Rat
PMID	Dilution	Publication
32910475	1:1,000	Nakamura, D.S., et al. 2020. Mitochondrial Dynamics and Bioenergetics Regulated by Netrin-1 in Oligodendrocytes.. Glia, 392-412.
23044338	1:200	Daadi, M.M., et al. 2013. Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplantation, 881-892.
18286199	1:200	Daadi, M.M., et al. 2008. Adherent self-renewable human embryonic stem cell-derived neural stem cell line: functional engraftment in experimental stroke model. PLoS One, e1644.

Immunohistochemistry: Rat
PMID	Dilution	Publication
22907787	1:250	Buller, B., et al. 2012. Regulation of serum response factor by miRNA‐200 and miRNA‐9 modulates oligodendrocyte progenitor cell differentiation. Glia, 1906-1914.
18286199	1:200	Daadi, M.M., et al. 2008. Adherent self-renewable human embryonic stem cell-derived neural stem cell line: functional engraftment in experimental stroke model. PLoS One, e1644.

Western Blot: Mouse
PMID	Dilution	Publication
28484008	not listed	Beirowski, B., et al. 2017. mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells. PNAS USA, E4261-E4270.

Western Blot: Rat
PMID	Dilution	Publication
18286199	1:200	Daadi, M.M., et al. 2008. Adherent self-renewable human embryonic stem cell-derived neural stem cell line: functional engraftment in experimental stroke model. PLoS One, e1644.

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Anti-CNPase (Cyclic Nucleotide Phosphodiesterase) Antibody

SKU: CNP

Product Details

Product Specific References for Applications and Species

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