Anti-Casein kinase I isoform alpha (CKI-alpha) Antibody
Our Anti-Casein kinase I isoform alpha (CKI-alpha) chicken polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Casein kinase I isoform alpha (CKI-alpha), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, WB.
A: Immunofluorescence analysis of casein kinase 1 alpha expression (red) in HeLa cells with C-1371-100. Casein kinase 1 alpha immunoreactivity is observed in the nucleus and the cytoplasm. Nuclear pore complexes (green) were stained with monoclonal antibody M-1397-250. Cell nuclei were visualized with Hoechst dye (blue).
B: Western blot of whole rat spinal cord homogenate. C-1371-100 (1:10,000 dilution) detects 2 bands at apparent molecular weights of ~38 kDa and ~34 kDa, corresponding to CK1 alpha and related isotypes.
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B: Western blot of whole rat spinal cord homogenate. C-1371-100 (1:10,000 dilution) detects 2 bands at apparent molecular weights of ~38 kDa and ~34 kDa, corresponding to CK1 alpha and related isotypes.
Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
ICC, IHC, WB
Chicken
SKU: C-1371-100
Product Details
Casein kinase I isoform alpha (CKI-alpha)
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
IgY preparation
Polyclonal
IgY
ICC, IHC, WB
Chicken
38 kDa
Shortest isoform of recombinant full length CK1a.
Human
Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Lyophilized IgY preparation, with sodium azide.
WB: 1:5000-1:10000
ICC: 1:500-1:1000
ICC: 1:500-1:1000
Unconjugated
Antibody recognizes CK1 by Western blot and immunohistochemistry. Cross reactivity.
For research use only.
United States
12 months after date of receipt (unopened vial).
CK1; CKI-alpha;
25°C (ambient)

