Anti-Casein, A2 beta Antibody

Name: Anti-Casein, A2 beta Antibody
Brand: Biosensis
SKU: C-1780-100
Price: 407.0 USD
Availability: InStock

Our Anti-Casein, A2 beta chicken polyclonal primary antibody detects bovine Casein, A2 beta, and is affinity-purified. It is validated for use in ELISA, WB.

Western Blot analysis of cow milk with chicken anti-A2 casein antibody (1 µg/mL). Blot was developed with donkey anti-chicken secondary antibody used at 1/4000 dilution. C-1780-100 is specific for A2 protein and does not recognize A1 protein on western blots.

Lane M: Molecular weight marker
Lane A1A1: 50 nL milk from the cow bearing A1/A1 alleles
Lane A1A2: 50 nL milk from the cow bearing A1/A2 alleles
Lane A2A2: 50 nL milk from the cow bearing A2/A2 alleles

Click on image to zoom

Species Reactivity

Bovine

Applications

ELISA, WB

Host Species

Chicken

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Data Sheet

SKU: C-1780-100

Volume/Size: 100 µL

Variant

Price:

$407.00

Quantity:

Product Details

Target

Casein, A2 beta

Scientific Background

Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, WB

Host Species

Chicken

Immunogen

A synthetic peptide (PGPIPNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A1 beta casein differs from bovine A2 beta casein by one amino acid (H82 -> P82)

Immunogen Species

Bovine

Species Reactivity

Bovine

Storage

Maintain unopened vial at -20°C for up to 12 months after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.

Physical State

Liquid

Production Notes

Affinity purified chicken IgY.

Buffer

PBS, pH 7.4, containing 0.02% sodium azide as preservative.

Dilution Ranges

WB: 1:200-1:1000

Application Details

The amount of milk per lane can be 0.05 µL-0.1 µL for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5 µL-1 µL) and add into 9.5 µL or 9 µL PBS and mix with 10 µL SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA.

Conjugate

Unconjugated

Specificity

The antibody is specific to A2 beta casein by Western blot. No cross-reactivity with A1 beta casein is seen. Species cross-reactivity not tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

P02666

Shipping

2-8°C (on cold packs)

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Anti-Casein, A2 beta Antibody

SKU: C-1780-100

Product Details

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