Anti-Ataxin-2 binding protein 1 (A2BP1/Fox1) Antibody (1G10)

Name: Anti-Ataxin-2 binding protein 1 (A2BP1/Fox1) Antibody
Brand: Biosensis
SKU: M-1688-100
Price: 327.0 USD
Availability: InStock

Our Anti-Ataxin-2 binding protein 1 (A2BP1) mouse monoclonal primary antibody detects human, mouse, and rat Ataxin-2 binding protein 1 (A2BP1), and is IgG. It is validated for use in IHC-Frozen, WB.

Extract from mouse brain. The antibody recognizes the ~48 kDa and 46 kDa proteins.

Rat brain cultures stained with M-1688-100 (green) and rabbit anti-NF-M (red, R-1395-50). The blue stain reveals DNA in the nuclei of these cells.

Extract from mouse brain. The antibody recognizes the ~48 kDa and 46 kDa proteins.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1688-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Ataxin-2 binding protein 1 (A2BP1)

Scientific Background

Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1). The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2). As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6).

Format

IgG

Clonality

Monoclonal

Clone

1G10

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Molecular Weight

46, 48 kDa

Immunogen

N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:2000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The antibody reacts with a 48 kDa and 46 kDa band by Western blot on extract from mouse brain. It has also been used successfully for immunocytochemistry on rat neuronal cultures.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

ataxin-2 binding protein 1 (A2BP1); RNA binding protein fox-1 homolog 1

UniProt ID

Q9NWB1

Shipping

25°C (ambient)

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Anti-Ataxin-2 binding protein 1 (A2BP1/Fox1) Antibody (1G10)

SKU: M-1688-100

Product Details

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