Anti-Ankyrin-G (Staining) Antibody (N106/36)

219 Citations

Our Anti-Ankyrin-G (Staining) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/36. It detects feline, hamster, human, mouse, non-human primate, and rat Ankyrin-G (Staining), and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Immunofluorescence staining of nodes of Ranvier in adult rat optic nerve with N106/36 (red) and Caspr rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Adult rat cortex immunohistochemistry of axon initial segments.
Adult rat thalamus immunohistochemistry of axon initial segments.
Immunostaining of mouse WT neurons (A) or neurons transfected with BFP vector (B), or BFP-Nfasc-shRNA (C) and stained for AnkG (red), Nfasc (green), and MAP2 (white). Image from publication CC-BY-4.0. PMID: 35850303
Immunostaining of V5-labeled Nav1.2 and Nav1.6 with MAP2 or AnkG in cultured rat hippocampal neurons. Image from publication CC-BY-4.0. PMID: 35672149
Immunostaining of of Nav1.2 and Nav1.6 labeled with V5 tag in mouse CA1 costained with AnkG(red). Arrowheads indicate Nav1.2 or Nav1.6-positive region with AnkG signals. DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35672149
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or somatodendritic marker MAP2. Image from publication CC-BY-4.0. PMID: 35966206
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or Kv7.3. Image from publication CC-BY-4.0. PMID: 35966206
Representative cortical layer 5/6 pyramidal neurons from control, (B) active MS lesion, (C) NAGM, (D) inactive MS lesion tissues immunolabelled with anti-SMI32, and their AIS immunolabelled with anti-AnkG. White rectangles in the merged channel panels indicate magnification areas and are presented in the lower panels. Dashed lines indicate the contour of the soma, arrowheads indicate the start of the AIS, and white lines represent soma-AIS gaps. Image from publication CC-BY-4.0. PMID:36451656
Representative micrographs showing immunohistochemical labelling for SSt (green), the AIS marker AnkyrinG (AnkG, magenta; cat. 75-146, 1:100), and their overlap (merge). The SSt soma is indicated with an asterisk (*) and the start and end of the AIS localised to that IN indicated (arrows). Image from publication CC-BY-4.0. PMID:36658491
Representative image of Alexa 555 (AL555, red) dye-filled RGC labeled for the axon initial segment (AIS) scaffolding protein ankyrin-G (AnkG, cat. 75-146, 1:200; green). Annotations demonstrate the dimensions of AIS distance from soma and length which were quantified. Image from publication CC-BY-4.0. PMID:37051140
Representative maximal projection of a confocal image of endogenous AnkG (cat. 75-146) staining in rat cortical neurons transfected with GFP with or without lithium stimulation. Image from publication CC-BY-4.0. PMID:36969554
Example images of ankG labeling (cat. 75-146, 1:200; green) in hRGCs (orange) treated with vehicle (A) or colchicine (B). Image from publication CC-BY-4.0. PMID:37010860
Immunostaining of mouse sliced optic nerve labeling Ankyrin G(Cat no 75-146, orange, 1:125) and Caspr (red). Nuclei are stained with DAPI. Image was taken at the CRL Molecular Imaging Center, RRID:SCR_017852, supported by the Helen Wills Neuroscience Institute. Image kindly provided by Anna Elleman, UC Berkley.
Representative confocal images showing the morphology of DIV21 primary hippocampal neurons over-producing mCherry (blue; circle highlights the complexity in panel 2, i.e. the number of processes that cross the circle is representative of the number of processes per cell) as well as EGFP–DHFR (−MTS; green; top panel) or Su9-EGFP–DHFR (+MTS; green; top panel) in the presence or absence of 100 nM MTX. Axons were stained with ankyrin-G (cat. 75-146, 1:400; magenta). CC-BY-4.0. PMID:37315917
CD59 (green) and ankyrin-G (AnkG, cat. 75-146; red) staining of WT murine teased fibers. Image from publication CC-BY-4.0. PMID:37875972
Immunofluorescence labeling of WT (left panels) and CD59 KO mouse(right panels) teased fibers show normal node structure by CD59 (green) and AnkG (cat. 75.146; red). Image from publication CC-BY-4.0. PMID:37875972
Reconstructions of the AIS (red) pooled from 3 independent experiments in 2 week wild-type (n = 27), iso-TDP-43G298S (n = 31), TDP-43G298S (n = 32), and C9orf72-3 (n = 21) MNs based on AnkG (cat. 75-146; red) immunofluorescence staining. Counterstained against CHR2-YFP (cyan). White arrows indicate start and end positionsImage from publication CC-BY-4.0. PMID: 38019651
Staining of axon initial segment (AIS) showing specific labeling of AnkyrinG (cat. 75-146, 1:250; red) in human iPSC-derived WT neuron. Nuclei stained with DAPI (blue). Image kindly provided by Yufei Liu, University of California, San Diego.
Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Immunofluorescence staining of axon initial segments in adult rat cortex with N106/36 (red) and bIV- spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Immunofluorescence staining of nodes of Ranvier in adult rat optic nerve with N106/36 (red) and Caspr rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).
Adult rat cortex immunohistochemistry of axon initial segments.
Adult rat thalamus immunohistochemistry of axon initial segments.
Immunostaining of mouse WT neurons (A) or neurons transfected with BFP vector (B), or BFP-Nfasc-shRNA (C) and stained for AnkG (red), Nfasc (green), and MAP2 (white). Image from publication CC-BY-4.0. PMID: 35850303
Immunostaining of V5-labeled Nav1.2 and Nav1.6 with MAP2 or AnkG in cultured rat hippocampal neurons. Image from publication CC-BY-4.0. PMID: 35672149
Immunostaining of of Nav1.2 and Nav1.6 labeled with V5 tag in mouse CA1 costained with AnkG(red). Arrowheads indicate Nav1.2 or Nav1.6-positive region with AnkG signals. DNA is dyed blue by Hoechst. Image from publication CC-BY-4.0. PMID: 35672149
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or somatodendritic marker MAP2. Image from publication CC-BY-4.0. PMID: 35966206
Cultured rat hippocampal neurons (DIV 11–12) were treated for 48 h with 1 μM TTX or its vehicle control (0.1% H2O) and subjected to immunocytochemistry with antibodies for the AIS marker ankyrin-G or Kv7.3. Image from publication CC-BY-4.0. PMID: 35966206
Representative cortical layer 5/6 pyramidal neurons from control, (B) active MS lesion, (C) NAGM, (D) inactive MS lesion tissues immunolabelled with anti-SMI32, and their AIS immunolabelled with anti-AnkG. White rectangles in the merged channel panels indicate magnification areas and are presented in the lower panels. Dashed lines indicate the contour of the soma, arrowheads indicate the start of the AIS, and white lines represent soma-AIS gaps. Image from publication CC-BY-4.0. PMID:36451656
Representative micrographs showing immunohistochemical labelling for SSt (green), the AIS marker AnkyrinG (AnkG, magenta; cat. 75-146, 1:100), and their overlap (merge). The SSt soma is indicated with an asterisk (*) and the start and end of the AIS localised to that IN indicated (arrows). Image from publication CC-BY-4.0. PMID:36658491
Representative image of Alexa 555 (AL555, red) dye-filled RGC labeled for the axon initial segment (AIS) scaffolding protein ankyrin-G (AnkG, cat. 75-146, 1:200; green). Annotations demonstrate the dimensions of AIS distance from soma and length which were quantified. Image from publication CC-BY-4.0. PMID:37051140
Representative maximal projection of a confocal image of endogenous AnkG (cat. 75-146) staining in rat cortical neurons transfected with GFP with or without lithium stimulation. Image from publication CC-BY-4.0. PMID:36969554
Example images of ankG labeling (cat. 75-146, 1:200; green) in hRGCs (orange) treated with vehicle (A) or colchicine (B). Image from publication CC-BY-4.0. PMID:37010860
Immunostaining of mouse sliced optic nerve labeling Ankyrin G(Cat no 75-146, orange, 1:125) and Caspr (red). Nuclei are stained with DAPI. Image was taken at the CRL Molecular Imaging Center, RRID:SCR_017852, supported by the Helen Wills Neuroscience Institute. Image kindly provided by Anna Elleman, UC Berkley.
Representative confocal images showing the morphology of DIV21 primary hippocampal neurons over-producing mCherry (blue; circle highlights the complexity in panel 2, i.e. the number of processes that cross the circle is representative of the number of processes per cell) as well as EGFP–DHFR (−MTS; green; top panel) or Su9-EGFP–DHFR (+MTS; green; top panel) in the presence or absence of 100 nM MTX. Axons were stained with ankyrin-G (cat. 75-146, 1:400; magenta). CC-BY-4.0. PMID:37315917
CD59 (green) and ankyrin-G (AnkG, cat. 75-146; red) staining of WT murine teased fibers. Image from publication CC-BY-4.0. PMID:37875972
Immunofluorescence labeling of WT (left panels) and CD59 KO mouse(right panels) teased fibers show normal node structure by CD59 (green) and AnkG (cat. 75.146; red). Image from publication CC-BY-4.0. PMID:37875972
Reconstructions of the AIS (red) pooled from 3 independent experiments in 2 week wild-type (n = 27), iso-TDP-43G298S (n = 31), TDP-43G298S (n = 32), and C9orf72-3 (n = 21) MNs based on AnkG (cat. 75-146; red) immunofluorescence staining. Counterstained against CHR2-YFP (cyan). White arrows indicate start and end positionsImage from publication CC-BY-4.0. PMID: 38019651
Staining of axon initial segment (AIS) showing specific labeling of AnkyrinG (cat. 75-146, 1:250; red) in human iPSC-derived WT neuron. Nuclei stained with DAPI (blue). Image kindly provided by Yufei Liu, University of California, San Diego.

Feline, Hamster, Human, Mouse, Non-Human Primate, Rat
ICC, IHC, IP, WB
Mouse
490
550
594
650

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Data Sheet
Trial Size Trial Size

SKU: 75-146

Volume: 100 µL
1-2 business days
Price:
$374.00

Product Specific References for Applications and Species

Immunocytochemistry: Hamster
PMID Dilution Publication
30219944not listedLeón-Espinosa, G., et al. 2018. Modifications of the axon initial segment during the hibernation of the Syrian hamster. Brain Structure and Function, 4307-4321.
Immunocytochemistry: Human
PMID Dilution Publication
358037341:200Fernandez Bessone, I., et al. 2022. DYRK1A Regulates the Bidirectional Axonal Transport of APP in Human-Derived Neurons. The Journal of Neuroscience, 6344-6358.
339457851:100James, O.G, et al. 2021. iPSC-derived myelinoids to study myelin biology of humans. Developmental Cell, 1346-1358.
31542321not listedSohn, P.D., et al. 2019. Pathogenic Tau Impairs Axon Initial Segment Plasticity and Excitability Homeostasis. Neuron, 458-470.
31091457not listedRhee, H.J., et al. 2019. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons. Cell Reports, 2212-2228.
310914561:1000Meijer, M., et al. 2019. A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons. Cell Reports, 2199-2211.
30219944not listedLeón-Espinosa, G., et al. 2018. Modifications of the axon initial segment during the hibernation of the Syrian hamster. Brain Structure and Function, 4307-4321.
28886366not listedAbernathy, D.G., et al. 2017. MicroRNAs Induce a Permissive Chromatin Environment that Enables Neuronal Subtype-Specific Reprogramming of Adult Human Fibroblasts. Cell Stem Cell, 332-348.
270017491:500Patzke, C., et al. 2016. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.. Journal of Experimental Medicine, 499-515.
Immunocytochemistry: Mouse
PMID Dilution Publication
38019651not listedHarley, P, et al. 2023. Aberrant axon initial segment plasticity and intrinsic excitability of ALS hiPSC motor neurons. Cell reports, 113509.
37428632not listedYoon, S, et al. 2023. Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome. Cell reports, 112784.
370108601:200Boal, A.M., et al. 2023. Microfluidic Platforms Promote Polarization of Human-Derived Retinal Ganglion Cells That Model Axonopathy. Translational Vision Science & Technology, 1.
358503031:1000He, L., et al. 2022. Crystal Structure of Ankyrin-G in Complex with a Fragment of Neurofascin Reveals Binding Mechanisms Required for Integrity of the Axon Initial Segment. The Journal of Biological Chemistry, 102272.
356721491:1000Liu, H., et al. 2022. Direct Observation of Compartment-Specific Localization and Dynamics of Voltage-Gated Sodium Channels. The Journal of Neuroscience, 5482-5498.
336896791:10Chang, K.J., et al. 2021. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells. . Elife, e64456.
32460012not listedYoon, S., et al. 2020. TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance. Cell Reports, 107685.
310687911:100Zhang, W., et al. 2019. P2Y1 Purinergic Receptor Modulate Axon Initial Segment Initial Development. Frontiers in Cellular Neuroscience, 152.
31042703not listedRojek, K.O., et al. 2019. Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS Biology, e3000253.
308726461:150Walton, C.C., et al. 2019. Primary neurons can enter M-phase. Scientific Reports, 4594.
308463101:000Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372.
303530931:100Wang, N., et al. 2018. The Sema3A receptor Plexin-A1 suppresses supernumerary axons through Rap1 GTPases. Scientific Reports, 15647.
302542841:150Barrio-Alonso, E., et al. 2018. Cell cycle reentry triggers hyperploidization and synaptic dysfunction followed by delayed cell death in differentiated cortical neurons. Scientific Reports, 14316.
30219944not listedLeón-Espinosa, G., et al. 2018. Modifications of the axon initial segment during the hibernation of the Syrian hamster. Brain Structure and Function, 4307-4321.
294316531:10Lin, T.W., et al. 2018. Regulation of Synapse Development by Vgat Deletion from ErbB4-Positive Interneurons. Journal of Neuroscience, 2533-2550.
292287861:200Clark, K., et al. 2017. Oxidative Stress Induces Disruption of the Axon Initial Segment. ASN Neuro, 1760000000000000.
283312031:100Rama, S., et al. 2017. The role of axonal Kv1 channels in CA3 pyramidal cell excitability. . Scientific reports, 44573.
278951201:50Klemens, C.A., et al. 2017. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). Journal of Biological Chemistry, 375-385.
264069151:2000Brügger, V., et al. 2015. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.. PLoS Biology, e1002258.
25362473not listedHo, T.S., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72.
24412576not listedBarry, J., et al. 2014. Ankyrin-G directly binds to kinesin-1 to transport voltage-gated Na+ channels into axons.. Developmental Cell, 117-31.
Immunocytochemistry: Rat
PMID Dilution Publication
373032351:400Needs, HI, et al. 2023. Aggregation Prone Tau Impairs Mitochondrial Import, Which Affects Organelle Morphology and Neuronal Complexity. Journal of Cell Science, jcs260993.
36969554not listedPiguel, N.H., et al. 2023. Palmitoylation Controls the Stability of 190 kDa Ankyrin-G in Dendritic Spines and is Regulated by ZDHHC8 and Lithium. Frontiers in Molecular Neuroscience, 1144066.
359662061:500Baculis, B.C., et al. 2022. Homeostatic Regulation of Extracellular Signal-Regulated Kinase 1/2 Activity and Axonal Kv7.3 Expression by Prolonged Blockade of Hippocampal Neuronal Activity. Frontiers in Cellular Neuroscience, 838419.
356721491:1000Liu, H., et al. 2022. Direct Observation of Compartment-Specific Localization and Dynamics of Voltage-Gated Sodium Channels. The Journal of Neuroscience, 5482-5498.
34260930not listedBuijs, R.R., et al. 2021. WDR47 protects neuronal microtubule minus ends from katanin-mediated severing. Cell Reports, 109371.
33838110not listedCross, J.A., et al. 2021. Fragment-linking peptide design yields a high-affinity ligand for microtubule-based transport. Cell Chemical Biology, 1347-1355.
331851901:200Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058.
33041849not listedTran, B., et al. 2020. Two KCNQ2 Encephalopathy Variants in the Calmodulin-Binding Helix A Exhibit Dominant-Negative Effects and Altered PIP 2 Interaction. Frontiers in Physiology, 1144.
325531551:20De Pace, R., et al. 2020. Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons. Cell Reports, 107775.
325425951:500Rahman-Enyart, A., et al. 2020. Neuregulins 1, 2, and 3 Promote Early Neurite Outgrowth in ErbB4-Expressing Cortical GABAergic Interneurons. Molecular Neurobiology, 3568-3588.
32179837not listedZhang, J., et al. 2020. Identifying mutation hotspots reveals pathogenetic mechanisms of KCNQ2 epileptic encephalopathy. Scientific Reports, 4756.
319348591:500Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
316036141:1000Boecket, C.A., et al. 2020. ToolBox: Live Imaging of intracellular organelle transport in induced pluripotent stem cell-derived neurons. Traffic, 138-155.
318629711:300Vassilopoulos, S., et al. 2019. Ultrastructure of the axonal periodic scaffold reveals a braid-like organization of actin rings. Nature Communications, 5803.
31042703not listedRojek, K.O., et al. 2019. Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS Biology, e3000253.
31036155not listedFletcher-Jones, A., et al. 2019. The C-terminal helix 9 motif in rat cannabinoid receptor type 1 regulates axonal trafficking and surface expression. Elife, e44252.
308820241:200Bakkum, D.J, et al. 2019. The Axon Initial Segment is the Dominant Contributor to the Neuron's Extracellular Electrical Potential Landscape. Advanced Biosystems, e1800308.
30784582not listedPan, X., et al. 2019. MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon. Cell Reports, 1988-1999.
30008368not listedKim, E. C., et al. 2018. Reduced axonal surface expression and phosphoinositide sensitivity in K v 7 channels disrupts their function to inhibit neuronal excitability in Kcnq2 epileptic encephalopathy. Neurobiology of Disease, 76-93.
29395909not listedBerger, S.L., et al. 2018. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Neuron, 555-570.
291804421:500Abiraman, K., et al. 2018. K Ca 2 channel localization and regulation in the axon initial segment. FASEB Journal, 1794-1805.
28533267not listedPinatel D, et al. 2017. The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to the axon initial segment in hippocampal neurons. Journal of Cell Science, 2209-2220.
284520881:500Evans, M.D., et al. 2017. Myosin II activity is required for structural plasticity at the axon initial segment. European Journal of Neuroscience, 1751-1757.
28231469not listedOkerlund, N.D., et al. 2017. Bassoon Controls Presynaptic Autophagy through Atg5. Neuron, 897-913.
268402081:100Hivert, B., et al. 2016. Assembly of juxtaparanodes in myelinating DRG culture: Differential clustering of the Kv1/Caspr2 complex and scaffolding protein 4.1B.. Glia, 840-852.
Immunohistochemistry: Feline
PMID Dilution Publication
334493751:250Deardoff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons. Journal of Physiology, 1391-1420.
Immunohistochemistry: Human
PMID Dilution Publication
364516561:200Senol, A.D., et al. 2022. Alterations Of The Axon Initial Segment In Multiple Sclerosis Grey Matter. Brain Communications, fcac284.
27956739not listedLopez, A.Y., et al. 2017. Ankyrin-G isoform imbalance and interneuronopathy link epilepsy and bipolar disorder. Molecular Psychiatry, 1464-1472.
Immunohistochemistry: Mouse
PMID Dilution Publication
37875972not listedKarbian, N, et al. 2023. Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system. Journal of Neuroinflammation, 245.
376508321:400Harris, AC, et al. 2023. Concussive head trauma deranges axon initial segment function in axotomized and intact layer 5 pyramidal neurons. Journal of Neurotrauma, 0.
370511401:200Boal, A.M., et al. 2023. Retinal Ganglion Cells Adapt to Ionic Stress in Experimental Glaucoma. Frontiers in Neuroscience, 1142668.
342264931:500Luo, B., et al. 2021. ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity. Translational Psychiatry, 361.
347855801:500Stevens, S.R., et al. 2022. Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival. Journal of Neuroscience, 44607.
34911751not listedKim, E. C., et al. 2021. Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2. PNAS: USA, .
346483301:500Lezmy, J., et al. 2021. Astrocyte Ca 2+-evoked ATP release regulates myelinated axon excitability and conduction speed. Science, eabh2858.
343481571:500Spratt, P.W.E., et al. 2021. Paradoxical hyperexcitability from Na V 1.2 sodium channel loss in neocortical pyramidal cells. Cell Reports, 109483.
341569841:200Wang, H.G., et al. 2021. Scn2a severe hypomorphic mutation decreases excitatory synaptic input and causes autism-associated behaviors. JCI Insight, e150698.
340381841:250Goethals, S., et al. 2021. Electrical match between initial segment and somatodendritic compartment for action potential backpropagation in retinal ganglion cells. Journal of Neurophysiology, 28-46.
338370491ug/mlIrie, T., et al. 2021. Essential Role of Somatic Kv2 Channels in High-Frequency Firing in Cartwheel Cells of the Dorsal Cochlear Nucleus. eNeuro, ENEURO.0515-20.2021.
336447711:1000Oliveira, L.S., et al. 2021. Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling. STAR protocols, 100336.
335637231:100Prades, S., et al. 2021. Functional P2X 7 Receptors in the Auditory Nerve of Hearing Rodents Localize Exclusively to Peripheral Glia. Journal of Neuroscience, 2615-2629.
334493751:250Deardoff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons. Journal of Physiology, 1391-1420.
332146551:300Rayi, P.R., et al. 2021. Chronic α1-Na/K-ATPase inhibition reverses the elongation of the axon initial segment of the hippocampal CA1 pyramidal neurons in Angelman syndrome model mice. Neuropsychopharmacology, 654-664.
329177081:200Werginz, P., et al. 2020. Tailoring of the axon initial segment shapes the conversion of synaptic inputs into spiking output in OFF-α T retinal ganglion cells. Neuroscience, .
327839271:500Booker, S.A., et al. 2020. Input-OutputRelationshipofCA1PyramidalNeurons Reveals Intact Homeostatic Mechanisms in a Mouse Model of Fragile X Syndrome. Cell Reports, 107988.
327363741:200Werginz, P., et al. 2020. The relationship between morphological properties and thresholds to extracellular electric stimulation in α RGCs. Journal of Neural Engineering, 45015.
324251571:500Liu, C.H., et al. 2020. β spectrin-dependent and domain specific mechanisms for Na + channel clustering. Elife, e56629.
32202512not listedZhou, X., et al. 2020. Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism. Journal of Clinical Investigation, 2220-2236.
320527421:500Liu, C.H., et al. 2020. Nodal β spectrins are required to maintain Na + channel clustering and axon integrity. Elife, e52378.
318136521:200Yoon, S., et al. 2020. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron, 506-521.
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
315901802ug/mlShiers, S., et al. 2020. Reversal of peripheral nerve injury-induced neuropathic pain and cognitive dysfunction via genetic and tomivosertib targeting of MNK. Neuropsychopharmacology, 524-533.
314536491:200Jha, M.K., et al. 2020. Monocarboxylate transporter 1 in Schwann cells contributes to maintenance of sensory nerve myelination during aging. Glia, 161-177.
319336261:1000Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
316360801:100Rovira-Esteban, L., et al. 2019. Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction. eNeuro, ENEURO.0220-19.2019.
316239181:1000D'Souza, R.D., et al. 2019. Spatial Clustering of Inhibition in Mouse Primary Visual Cortex. Neuron, 588-600.
316117771:200Raghuram, V., et al. 2019. Scaling of the AIS and Somatodendritic Compartments in α S RGCs. Frontiers in Cellular Neuroscience, 436.
312915701:500Steinecke, A., et al. 2019. In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9. Cell Reports, 325-331.
308463101:500Tai, Y., et al. 2019. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron, 358-372.
307736331:2000Boccalaro, I.B., et al. 2019. Cell type-specific distribution of GABA A receptor subtypes in the mouse dorsal striatum. Journal of Comparative Neurology, 2030-2046.
306513271:1000Bolós, M., et al. 2019. Maturation Dynamics of the Axon Initial Segment (AIS) of Newborn Dentate Granule Cells in Young Adult C57BL/6J Mice. Journal of Neuroscience, 1605-1620.
293008911:500Scott, R., et al. 2019. Loss of Cntnap2 Causes Axonal Excitability Deficits, Developmental Delay in Cortical Myelination, and Abnormal Stereotyped Motor Behavior. Cereb Cortex, 586-597.
30104343not listedFavero, M., et al. 2018. A Transient Developmental Window of Fast-Spiking Interneuron Dysfunction in a Mouse Model of Dravet Syndrome. Journal of Neuroscience, 7912-7927.
300304042ug/mlShiers, S., et al. 2018. Neuropathic Pain Creates an Enduring Prefrontal Cortex Dysfunction Corrected by the Type II Diabetic Drug Metformin But Not by Gabapentin. Journal of Neuroscience, 7337-7350.
296762601:500Galliano, E., et al. 2018. Embryonic and postnatal neurogenesis produce functionally distinct subclasses of dopaminergic neuron. Elife, e32373.
294761051:200Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
293540331:1000Wang, T., et al. 2018. Enhanced Transmission at the Calyx of Held Synapse in a Mouse Model for Angelman Syndrome. Frontiers in Cellular Neuroscience, 418.
29282769not listedBang., M.L, et al. 2018. Glial M6B stabilizes the axonal membrane at peripheral nodes of Ranvier. Glia, 801-812.
289228601:250Schlüter, A., et al. 2017. Structural Plasticity of Synaptopodin in the Axon Initial Segment during Visual Cortex Development. Cerebral Cortex, 4662-4675.
289121621:500Ishino, Y., et al. 2017. Regional Cellular Environment Shapes Phenotypic Variations of Hippocampal and Neocortical Chandelier Cells. Journal of Neuroscience, 9901-9916.
28815501not listedLu, Q., et al. 2017. Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines. Methods of Molecular Biology, 181-194.
286765741:500Ottolini, M., et al. 2017. Aberrant Sodium Channel Currents and Hyperexcitability of Medial Entorhinal Cortex Neurons in a Mouse Model of SCN8A Encephalopathy.. Journal of Neuroscience, 7643-7655.
285848771:500Steinecke, A., et al. 2017. Neocortical Chandelier Cells Developmentally Shape Axonal Arbors through Reorganization but Establish Subcellular Synapse Specificity without Refinement. eNeuro, .
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281193451:500Minge, D., et al. 2017. Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination. Cerebral Cortex, 903-918.
27956739not listedLopez, A.Y., et al. 2017. Ankyrin-G isoform imbalance and interneuronopathy link epilepsy and bipolar disorder. Molecular Psychiatry, 1464-1472.
274482451 : 50Serwanski, D.R., et al. 2017. Heterogeneity of astrocyte and NG2 cell insertion at the node of ranvier.. Journal of Comparative Neurology, 535-552.
273568711:1000Sohn, P.D., et al. 2016. Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.. Molecular Neurodegeneration, e13073.
Immunohistochemistry: Non-Human Primate
PMID Dilution Publication
286246161:500Atapour, N., et al. 2017. Age-related plasticity of the axon initial segment of cortical pyramidal cells in marmoset monkeys. Neurobiology of Aging, 95-103.
Immunohistochemistry: Rat
PMID Dilution Publication
366584911:1000Yang, Y., et al. 2023. Identifying Foetal Forebrain Interneurons as a Target for Monogenic Autism Risk Factors and the Polygenic 16p11.2 Microdeletion. BMC Neuroscience, 5.
347994471:100Zbili, M., et al. 2021. Homeostatic regulation of axonal Kv1. 1 channels accounts for both synaptic and intrinsic modifications in the hippocampal CA3 circuit. . Proceedings of the National Academy of Sciences, e2110601118.
340003001:500Kawai, T., et al. 2021. Heterologous functional expression of ascidian Nav1 channels and close relationship with the evolutionary ancestor of vertebrate Nav channels. Journal of Biological Chemistry, 100783.
338903331:100Zetter, M.A., et al. 2021. Microglial synaptic pruning on axon initial segment spines of dentate granule cells: Sexually dimorphic effects of early-life stress and consequences for adult fear response. Journal of Neuroendocrinology, e12969.
336447711:1000Oliveira, L.S., et al. 2021. Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling. STAR protocols, 100336.
334493751:250Deardoff, A.S., et al. 2021. Location, location, location: the organization and roles of potassium channels in mammalian motoneurons. Journal of Physiology, 1391-1420.
325531161:100Hanemaaijer, N.A., et al. 2020. Ca 2+ entry through Na V channels generates submillisecond axonal Ca 2+ signaling. Elife, .
319348591:500Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
28673977not listedSeagar, M., et al. 2017. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels. Proceedings of the National Academy of Sciences, 114(29), pp.7719-7724.. Proceedings of the National Academy of Sciences, 7719-7724.
274587971 : 1000Sun, Y., et al. 2016. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients.. Elife, .
25362471not listedChang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-1681.
Immunoprecipitation: Mouse
PMID Dilution Publication
25362471not listedChang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-1681.
Immunoprecipitation: Rat
PMID Dilution Publication
25362471not listedChang, K.J., et al. 2014. Glial ankyrins facilitate paranodal axoglial junction assembly.. Nature Neuroscience, 1673-1681.
STORM: Rat
PMID Dilution Publication
319348591:50Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
Western Blot: Human
PMID Dilution Publication
270017491:500Patzke, C., et al. 2016. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.. Journal of Experimental Medicine, 499-515.
Western Blot: Mouse
PMID Dilution Publication
33398084not listedYoon, S., et al. 2021. Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome. Molecular Psychiatry, 1775-1789.
31910261not listedLee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033.
31813652not listedYoon, S., et al. 2020. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron, 506-521.
319336261:500Alshammari, M.A., et al. 2019. Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model. Neural Plasticity, 4893103.
25362473not listedHo, T.S., et al. 2015. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier.. Nature Neuroscience, 1664-72.
250937261:1000Bosch, M.K., et al. 2014. Dual transgene expression in murine cerebellar Purkinje neurons by viral transduction in vivo.. PLoS One, e104062.
24412576not listedBarry, J., et al. 2014. Ankyrin-G directly binds to kinesin-1 to transport voltage-gated Na+ channels into axons.. Developmental Cell, 117-31.
239112851:1000Kaphzan, H., et al. 2013. Genetic reduction of the α1 subunit of Na/K-ATPase corrects multiple hippocampal phenotypes in Angelman syndrome.. Cell Report, 405-412.
Western Blot: Rat
PMID Dilution Publication
319348591:2000Yang, H.Q., et al. 2020. Ankyrin-G mediates targeting of both Na + and K ATP channels to the rat cardiac intercalated disc. Elife, e52373.
Additional Publications: Unspecified
PMID Publication
30951495Abouelezz, A., et al. 2019. Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin. Biological Chemistry, 1141-1146.
27452469Yang, S., et al. 2016. β-Arrestin-Dependent Dopaminergic Regulation of Calcium Channel Activity in the Axon Initial Segment. Cell Reports, 1518-1526.
27369547Singh, P., et al. 2016. Multiscale Analysis of Neurite Orientation and Spatial Organization in Neuronal Images. Neuroinformatics, 465-477.
27053216Darbelli, L., et al. 2016. Quaking Regulates Neurofascin 155 Expression for Myelin and Axoglial Junction Maintenance. Journal of Neuroscience, 4106-4120.
26220974Gong, X., et al. 2015. Activating the translational repressor 4E-BP or reducing S6K-GSK3β activity prevents accelerated axon growth induced by hyperactive mTOR in vivo.. Human Molecular Genetics, 5746-5758.
26007637Abidi, A., et al. 2015. A recurrent KCNQ2 pore mutation causing early onset epileptic encephalopathy has a moderate effect on M current but alters subcellular localization of Kv7 channels.. Neurobiology of Disease, 80-92.
26001168Horschitz, S., et al. 2015. Impact of preconditioning with retinoic acid during early development on morphological and functional characteristics of human induced pluripotent stem cell-derived neurons.. Stem Cell Research, 30-41.
25950943Papandréou, M.J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-537.
25874799Akin, E.J., et al. 2015. Preferential targeting of Nav1.6 voltage-gated Na+ Channels to the axon initial segment during development.. PLoS One, .
25788676Liu, Y., et al. 2015. Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration.. Journal of Neuroscience, 4587-4598.
25653382Baalman, K., et al. 2015. Axon initial segment-associated microglia.. Journal of Neuroscience, 2283-2292.
25632134Chand, A.N., et al. 2015. A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.. Journal of Neuroscience, 1573-1590.
25561543Freeman, S.A., et al. 2015. Acceleration of conduction velocity linked to clustering of nodal components precedes myelination.. PNAS: USA, E321-328.
25421039Wimmer, V.C., et al. 2015. Sodium channel β1 subunit localizes to axon initial segments of excitatory and inhibitory neurons and shows regional heterogeneity in mouse brain.. Journal of Comparative Neurology, 814-830.
24610121Del Puerto, A., et al. 2015. ATP-P2X7 Receptor Modulates Axon Initial Segment Composition and Function in Physiological Conditions and Brain Injury.. Cereb Cortex, 2282-2294.
24275828Forro, T., et al. 2015. Temporal organization of GABAergic interneurons in the intermediate CA1 hippocampus during network oscillations.. Cereb Cortex, 128-1240.

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