Anti-Alpha-internexin (Alpha-Inx) Antibody

Name: Anti-Alpha-internexin (Alpha-Inx) Antibody
Brand: Biosensis
SKU: C-1377-50
Price: 327.0 USD
Availability: InStock

Our Anti-Alpha-internexin (Alpha-Inx) chicken polyclonal primary antibody detects cat, human, mouse, other mammals (predicted), and rat Alpha-internexin (Alpha-Inx), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, WB.

Mixed neuron glia cultures stain with Chicken polyclonal antibody to Internexin alpha C-1377-50 (red) and counterstained with Rabbit polyclonal antibody to Coronin 1a: Whole serum, R-1335-50 (green) which is an excellent marker of microglia and lymphocytes. This internexin antibody is an excellent marker of neuronal processes in these cultures.

Left: Fluorescent staining of rat cerebellum section by Immunohistochemistry with chicken antibody to alpha-internexin (1:5,000, red), and co-stained with mouse antibody to MBP (M-1384-50, 1:5,000, green). IHC Method: Following transcardial perfusion with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons. Right: Western blot analysis of alpha-internexin expression in tissue lysates. [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, [7] pig spinal cord. The antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa, with some variability among different species. Antibody dilution: 1:10,000.

Mixed neuron glia cultures stain with Chicken polyclonal antibody to Internexin alpha C-1377-50 (red) and counterstained with Rabbit polyclonal antibody to Coronin 1a: Whole serum, R-1335-50 (green) which is an excellent marker of microglia and lymphocytes. This internexin antibody is an excellent marker of neuronal processes in these cultures.

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Species Reactivity

Feline, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1377-50

Volume/Size: 50 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Alpha-internexin (Alpha-Inx)

Scientific Background

Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

64-66 kDa

Immunogen

Recombinant rat alpha-internexin expressed and purified from E. coli

Immunogen Species

Rat

Species Reactivity

Feline, Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, rat, mouse, feine. It is predicted to react with other mammals.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

66 kDa neurofilament protein; NF-66; Neurofilament-66; Alpha-internexin; Alpha-Inx; Neurofilament 5; INA; NEF5;

UniProt ID

P23565

Shipping

25°C (ambient)

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Anti-Alpha-internexin (Alpha-Inx) Antibody

SKU: C-1377-50

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