Brain-validated SARM1 and CSPG4/NG2 monoclonal antibodies now available

Contributed by Amy Archuleta

Great research begins with great tools. We’ve extensively validated our new SARM1 and CSPG4/NG2 monoclonal antibodies on brain tissue to ensure they perform as intended. Available in full, trial, and bulk sizes as well as fluorophore-conjugated options to meet all of your research needs.

SARM1 is a key player in neurodegeneration and neurodegenerative disease.

SARM1 (Sterile Alpha and TIR Motif-Containing 1) is a protein that plays an important role in the process of axon degeneration. When an axon is injured or stressed, SARM1 triggers a series of biochemical events leading to the dismantling of the damaged axon. This process, known as Wallerian degeneration, is crucial for the removal of debris and regeneration of the injured neuron. Understanding the function of SARM1 has implications for developing therapeutic interventions to prevent or slow down axon degeneration in neurodegenerative diseases such as Alzheimer's, Parkinson's, and ALS, as well as traumatic nerve injuries.

Our SARM1 antibody is highly-specific and perfect for Western blot, IHC, and ICC.

ICC staining in COS-7 cells shows 100% correspondence between mouse anti-SARM1 signal and anti-flag in transfected cells:

Staining of COS-7 cells expressing SARM1-flag using Antibodies Incorporated mouse anti-SARM1 clone 6174-11 (green) and rabbit anti-flag (red). DAPI nuclear stain (blue) showing nuclei of both transfected and untransfected cells.

IHC showing strong staining for SARM1 in the Purkinje layer of the cerebellum:

Sagittal section of formalin-fixed, paraffin-embedded rat brain stained with Antibodies Incorporated mouse anti-SARM1 (clone 6174-11) antibody at 1:500.

Western blot showing prominent SARM1 band at 73 kDa:

77-522 WB
Western blotting of rat, human, or mouse brain homogenate (20 ug/lane) and transfected COS7 cell lysates (5 ug/ml) with Antibodies Incorporated mouse anti-SARM1 antibody (clone 6174-11). Overexpressed SARM1 in lane 4 runs higher due to dual MYC-FLAG tag.

CSPG4/NG2 is a protein relevant to studies in both neurological diseases and cancer.

CSPG4 (Chondroitin Sulfate Proteoglycan 4), also known as NG2 (Neuron-Glial Antigen 2), is a protein that plays an important role in the central nervous system, where it is crucial for the development and differentiation of OPCs into mature oligodendrocytes. These cells are responsible for producing myelin, the protective coating of nerve fibers. CSPG4/NG2 has also been implicated in cancer progression and metastasis. Research on the CSPG4/NG2 protein may help develop both neurological disorder therapies and cancer treatment strategies.

Our CSPG4/NG2 antibody works great in IHC and Western blot.

IHC staining is apparent in multiple rat brain regions, including cerebellum, hippocampus, and cortex.

Sagittal section of formalin-fixed, paraffin-embedded rat brain: cerebellum, hippocampus and cortex (A-C respectively). Sections were stained with Antibodies Incorporated mouse anti-CSPG4/NG2 (clone 6203-34) antibody at 1:40.

Western blots show a strong single band at 250 kDa:

Western blotting of mouse or rat brain homogenate (10 ug/lane) with Antibodies Incorporated anti-CSPG4/NG2 antibody at 1:50.

Designed, developed, and manufactured in Davis, California. Making exceptional tools for exceptional science. Need a reagent we don’t have? Connect with us to collaborate.