ICT Answers Your Questions!

We love hearing from you with questions regarding our products!

Read below for a question regarding our Acridine Orange Staining Solution:

Question: Could you please let me know if this acridine orange could be used to visualize RNA and DNA separately in agarose gel electrophoresis? It would be greatly appreciated if you could give us some advice. What kind of protocol could be recommended?

Answer: Thank you for contacting ICT. We are very happy to help you.
Acridine Orange (AO) is a fluorescent cationic dye that can be used to visualize both DNA and RNA in agarose gels and polyacrylamide gels. But it is predominantly used in Fluorescence Microscopy and Flow Cytometry.

We supplied AO ready to use at 1 mM concentration for Fluorescence assays. For gel electrophoresis, we would recommend diluting AO in either water or buffer to 4 - 5 uM and trying it out for start. You might have to try out varying concentrations of AO to figure out optimum AO concentration with minimum gel background staining.

We hope the above information is helpful!

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If you have any questions regarding our products, please email help@immunochemistry.com