Iba1/AIF1: An excellent marker for microglia/macrophages

Introducing our Iba1/AIF1 chicken polyclonal antibody for use in both immunostaining and blotting. Developed and produced by Aves Labs’ scientists in Davis, California.

Contributed by Kameron Simpson



Iba1/AIF1 is a highly conserved scaffold/adaptor protein often associated with inflammatory diseases.

Iba-1, Ionized calcium binding adaptor molecule 1 (also known as, AIF1, allograft inflammatory factor-1) is a 17 kDa cytosolic protein that binds calcium and actin, with its expression induced by cytokines such as IFN-γ. The presence of Iba1/AIF1 is associated with inflammation due to increased levels of the protein observed in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Its activation in macrophages implicates Iba1/AIF1 in several diseases such as kidney disease, rheumatoid arthritis, cancer, atherosclerosis, metabolic diseases, and neurological disorders. (Ortega, M.A. et al 2023). It is also considered a marker of activated human vascular smooth muscle cells and arterial injury (Vizioli J., et al, 2020).

Recent research correlates AIF1 expression with the susceptibility and progression of various diseases, potentially making AIF1/Iba1 a groundbreaking biomarker for predicting prognosis and assessing treatment response (Ortega, M.A. et al 2023).

Iba1/AIF4 WB

Check out the most recent publications using our Iba1/AIF1 antibody below!

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse
Immunohistochemistry: Mouse
PMID Dilution Publication
36405424 not listed Carpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
35633501 not listed Galloway, D.A., et al. 2022. Investigating the NLRP3 Inflammasome and its Regulator miR-223-3p in Multiple Sclerosis and Experimental Demyelination. Journal of Neurochemistry, 94-112.