Products: Primary Antibodies

Anti-VIP Antibody (L108/92)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-447	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-447	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Rat

Applications:

IHC

Target Description:

Vasoactive Intestinal Peptide (VIP) or PHM27 is encoded by the gene VIP and belongs to the glucagon family. VIP is a secreted peptide hormone and binds to the VIP receptors VIP R1 and VIP R2. Signaling through the receptors, VIP is involved diverse processes including vasodilation, regulation of blood pressure, stimulates myocardial contractility and neurotransmission. VIP is produced in many tissues including gut, pancreas, heart and brain. Diseases associated with this gene include Vipoma and Vasomotor Rhinitis.

Immunogen:

Fusion protein amino acids 81-152 (mature protein) of human VIP (also known as Vasoactive intestinal peptide and Vasoactive intestinal polypeptide, cleaved into Intestinal peptide PHI-42 / PHV-42 for Peptide histidine valine 42 and Intestinal peptide PHM-27 for Peptide histidine methioninamide 27 / Intestinal peptide PHI-27 for Peptide histidine isoleucinamide 27, accession number P01282)
Rat: 92% identity (66/72 amino acids identical)
Mouse: 90% identity (65/72 amino acids identical)
50% identity with PACAP

Protein Name(s):

VIP peptides [Cleaved into: Intestinal peptide PHV-42 (Peptide histidine valine 42); Intestinal peptide PHM-27 (Peptide histidine methioninamide 27); Vasoactive intestinal peptide (VIP) (Vasoactive intestinal polypeptide)]

Gene Name(s):

VIP

Antibody Registry ID:

Purified: AB_2651163
TC Supernatant: AB_ 2619640

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.