Products: Primary Antibodies

Anti-TrpC4 Antibody (N77/15)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-119	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-119	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC IP WB

Target Description:

Short transient receptor potential channel 4 or TrpC4 is encoded by the gene TrpC4. TrpC4 belongs to the transient receptor family, a group of membrane bound ion channels that have a relatively non-selective permeability to cations, including sodium, calcium and magnesium. This protein is thought to have a role as a cell-cell contact-dependent endothelial calcium entry channel. Trpc4 is found in many tissues including CNS, placenta, heart, smooth muscle, testis, kidney and brain. Mutations in this gene areassociated with generalized epilepsy with photosensitivity.

Immunogen:

Synthetic peptide amino acids 930-947 (FKSEKVVVEDTVPIIPKE) of rat TrpC4 (also known as short transient receptor potential channel 4, Trp4, capacitative calcium entry channel 1 and CCE1, accession number O35119); Mouse: 100% identity (18/18 amino acids identical); Human: 100% identity (18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

TrpC4

Gene Name(s):

Trpc4

Antibody Registry ID:

Purified: AB_2256454
TC Supernatant: AB_10698036

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

110 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.