Products: Primary Antibodies

Anti-TrpC4 (N77/15)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-119	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Catalog # 73-119	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC IP WB

Immunogen:

Synthetic peptide amino acids 930-947 (FKSEKVVVEDTVPIIPKE) of rat TrpC4 (also known as short transient receptor potential channel 4, Trp4, capacitative calcium entry channel 1 and CCE1, accession number O35119)
Mouse: 100% identity (18/18 amino acids identical)
Human: 100% identity (18/18 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Short transient receptor potential channel 4 (Trp4) (TrpC4) (Capacitative calcium entry channel 1) (CCE1)

Gene Name(s):

Trpc4

Antibody Registry ID:

Purified: AB_2256454
TC Supernatant: AB_10698036

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

110 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Menon RP, Soong D, de Chiara C, Holt MR, Anilkumar N, Pastore A.. (2012), 'The importance of serine 776 in Ataxin-1 partner selection: a FRET analysis. .' Scientific Reports. 10.1038/srep00919.
Jafar-Nejad P, Ward CS, Richman R, Orr HT, Zoghbi HY.. (2011), 'Regional rescue of spinocerebellar ataxia type 1 phenotypes by 14-3-3epsilon haploinsufficiency in mice underscores complex pathogenicity in neurodegeneration. .' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1018748108.
Zhang C, Browne A, Child D, Divito JR, Stevenson JA, Tanzi RE.. (2010), 'Loss of function of ATXN1 increases amyloid beta-protein levels by potentiating beta-secretase processing of beta-amyloid precursor protein. .' The Journal of Biological Chemistry. 10.1074/jbc.M109.079079.
de Chiara C, Menon RP, Strom M, Gibson TJ, Pastore A.. (2009), 'Phosphorylation of S776 and 14-3-3 binding modulate ataxin-1 interaction with splicing factors. .' PLoS One. 10.1371/journal.pone.0008372.
Zhai RG, Zhang F, Hiesinger PR, Cao Y, Haueter CM, Bellen HJ.. (2008), 'NAD synthase NMNAT acts as a chaperone to protect against neurodegeneration. .' Nature. 10.1038/nature06721.