Products: Primary Antibodies

Anti-TRIP8b (constant) (N212/7)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-243	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-243	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 1-192 (exon 1a, exon 4 and constant region) of rat TRIP8b (also known as TPR-containing Rab8b-interacting protein, Peroxisome biogenesis factor 5-like, Peroxin-5 or PEX5-related/like protein, Pex5Rp, Pex5l, Pex5r, Pex2, Pxr2, accession number Q925N3)
Mouse: 97% identity (147/151 amino acids identical)
Human: 83% identity (180/216 amino acids identical)

Cross Reactivity:

Cross-reacts with multiple TRIP8b isoforms

Protein Name(s):

PEX5-related protein (PEX5-like protein) (Peroxin-5-related protein) (TPR-containing Rab8b-interacting protein) (Tetratricopeptide repeat-containing Rab8b-interacting protein) (Pex5Rp) (TRIP8b)

Gene Name(s):

Pex5l Pex2 Pex5r Trip8b

Antibody Registry ID:

Purified: AB_10675329
TC Supernatant: AB_10698034

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Cao-Ehlker X1, Zong X, Hammelmann V, Gruner C, Fenske S, Michalakis S, Wahl-Schott C, Biel M.. (2013), 'Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3)..' J Biol Chem.. 10.1074/jbc.M112.434803.