Products: Primary Antibodies

Anti-TMEM119 Antibody (L128/43)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

★ Trial Size Available ★
Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-512
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$285.00
Brand
NeuroMab Logo
Catalog #
75-512-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$119.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC

Target Description:

TMEM119, is a plasma membrane protein first identified because of its role in the differentiation of osteoblasts (Kanamoto et al., 2009). However TMEM119 is also known to be a specific marker of microglia, the tissue-resident macrophages of brain and spinal cord (Bennett et al., 2016). Unlike other microglia markers, TMEM119 expression readily distinguishes resident microglia from blood-derived macrophages, making it a highly specific microglia marker. In healthy brain microglia are the resident immune cells where they act to neutralize pathogens, clear dead and dying cells, and prune synaptic connections. However, in neurodegenerative disease microglia can actually promote neuronal degeneration through reduced phagocytic clearance and increased pro-inflammatory effects. Our anti-TMEM119 antibody is a highly specific microglial marker for mouse TMEM119 and works by western blot and immunocytochemistry.

Immunogen:

Fusion protein amino acids 113-280 (cytoplasmic C-terminus) of mouse Tmem119.

Protein Name(s):

TMEM119, OBIF

Gene Name(s):

TMEM119


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

30 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.