Products: Primary Antibodies

Anti-Pan-SAPAP (N127/31)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-156	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-156	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 772-992 (C-terminus) of rat SAPAP1 (also known as disks large-associated protein 1, guanylate kinase-associated protein, GKAP, SAP90/PSD-95-associated/binding protein 1, accession number P97836)

Mouse: 99% identity (200/201 amino acids identical)
Human: 99% identity (199/201 amino acids identical)
55-65% identity with other SAPAPs

Cross Reactivity:

Cross-reacts with other SAPAPs

Protein Name(s):

Disks large-associated protein 1 (SAPAP1) SAPAP-1 Disks large-associated protein 2 (SAPAP2) SAPAP-2 Disks large-associated protein 3 (SAPAP3) SAPAP-3 Disks large-associated protein 4 (SAPAP-4) SAPAP4

Gene Name(s):

DLGAP1 DAP1 GKAP DLGAP2 C8orf68 DAP2 ERICH1-AS1 DLGAP3 DAP3 DLGAP4 DAP4 KIAA0964 SAPAP4

Antibody Registry ID:

Purified: AB_10671947
TC Supernatant: AB_10697886

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

120 kDa (SAPAP2), 110 kDa (SAPAP1/3/4)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Xu J, Marshall JJ, Fernandes HB, Nomura T, Copits BA, Procissi D, Mori S, Wang L, Zhu Y, Swanson GT, Contractor A.. (2017), 'Complete Disruption of the Kainate Receptor Gene Family Results in Corticostriatal Dysfunction in Mice. .' Cell Report. 10.1016/j.celrep.2017.01.073.
Wang X, Bey AL, Katz BM, Badea A, Kim N, David LK, Duffney LJ,, Kumar S, Mague SD, Hulbert SW, Dutta N, Hayrapetyan V, Yu C, Gaidis E, Zhao S, Ding JD, Xu Q,, Chung L, Rodriguiz RM, Wang F, Weinberg RJ, Wetsel WC,,,, Dzirasa K,, Yin H,, Jiang YH,,.. (), 'Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. .' Nature Communications. 10.1038/ncomms11459.
Zhang N, Zhong P, Shin SM, Metallo J, Danielson E, Olsen CM, Liu QS, Lee SH.. (2015), 'S-SCAM, a rare copy number variation gene, induces schizophrenia-related endophenotypes in transgenic mouse model. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3658-14.2015.
Braude JP, Vijayakumar S, Baumgarner K, Laurine R, Jones TA, Jones SM, Pyott SJ.. (), 'Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. .' Hearing Research. 10.1016/j.heares.2015.01.008.
Wei Z, Behrman B, Wu WH, Chen BS.. (2013), 'Subunit-specific regulation of N-methyl-D-aspartate (NMDA) receptor trafficking by SAP102 protein splice variants. .' The Journal of Biological Chemistry. 10.1074/jbc.M114.599969.
Han K, Holder JL Jr, Schaaf CP, Lu H, Chen H, Kang H, Tang J, Wu Z, Hao S, Cheung SW, Yu P, Sun H, Breman AM, Patel A, Lu HC, Zoghbi HY.. (), 'SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. .' Nature. 10.1038/nature12630.
Shin SM, Zhang N, Hansen J, Gerges NZ, Pak DT, Sheng M, Lee SH.. (2012), 'GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. .' Nature Neuroscience. 10.1038/nn.3259.
Danielson E, Zhang N, Metallo J, Kaleka K, Shin SM, Gerges N, Lee SH.. (2012), 'S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0025-12.2012.