Products: Primary Antibodies

Anti-Pan-MAGUK (K28/86)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-029	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-029	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with K28/86 (left) or N52A/42 (right) TC supe.$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus Zebrafish

Applications:

IP WB

Immunogen:

Fusion protein amino acids 77-299 of human PSD-95/SAP90

Rat: 100% identity (223/223 amino acids identical)
Mouse: 99% identity (221/223 amino acids identical)
65-80% identity with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102

Cross Reactivity:

Exhibits cross-reactivity against recombinant Chapsyn-110, SAP97 and SAP102

Protein Name(s):

Disks large homolog 1 (Synapse-associated protein 97) (SAP-97) (SAP97) (hDlg) Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93) Disks large homolog 3 (Neuroendocrine-DLG) (Synapse-associated protein 102) (SAP-102) (SAP102) (XLMR) Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Gene Name(s):

DLG1 DLG2 DLG3 KIAA1232 DLG4 PSD95

Antibody Registry ID:

Purified: AB_10673115
TC Supernatant: AB_10698179

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

95-110 kDa(varies with cell background due to phosphorylation)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

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Easley-Neal C, Fierro J Jr, Buchanan J, Washbourne P.. (2013), 'Late recruitment of synapsin to nascent synapses is regulated by Cdk5. .' Cell Report. 10.1016/j.celrep.2013.03.031.
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Chen X, Nelson CD, Li X, Winters CA, Azzam R, Sousa AA, Leapman RD, Gainer H, Sheng M, Reese TS.. (2011), 'PSD-95 is required to sustain the molecular organization of the postsynaptic density. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5968-10.2011.
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Sharma N, Deppmann CD, Harrington AW, St Hillaire C, Chen ZY, Lee FS, Ginty DD.. (2010), 'Long-distance control of synapse assembly by target-derived NGF..' Neuron. 10.1016/j.neuron.2010.07.018.
Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L.. (2010), 'Visualizing the distribution of synapses from individual neurons in the mouse brain..' PLoS One. 10.1371/journal.pone.0011503.
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