Products: Primary Antibodies

Anti-Pan-MAGUK Antibody (K28/86)

from NeuroMab

Product Summary:

This antibody recognizes endogenous levels of DLG1/SAP97, DLG2/Chapsyn-110, DLG3/SAP102, and DLG4/PSD95 by western blot, immunoprecipitation, and immunocytochemistry.

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-029	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-029	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with K28/86 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus Zebrafish

Applications:

IP WB

Target Description:

Membrane-associated guanylate kinases (MAGUKs) are a large family of scaffold proteins that play essential roles in tissue development, cell-cell communication, cell polarity control, and cellular signal transduction. MAGUKs act as key scaffolds in surface complexes containing receptors, adhesion proteins, and various signaling molecules and play key roles in cell-cell intercommunication. The neuronal synapse is a prime example of this, where the MAGUK proteins play a critical role in organizing both pre and post-synaptic proteins into functional assemblies. Major synaptic MAGUK proteins include PSD-95, SAP102, SAP97, PSD-93, CASK, and MAGIs. Together, they play roles in both synaptic development and in synaptic plasticity.

Immunogen:

Fusion protein amino acids 77-299 of human PSD-95/SAP90; Rat: 100% identity; Mouse=99% identity (221/223 amino acids)

Cross Reactivity:

Exhibits cross-reactivity against recombinant Chapsyn-110, SAP97 and SAP102

Protein Name(s):

Pan-MAGUK

Gene Name(s):

DLG1 DLG2 DLG3 KIAA1232 DLG4 PSD95

Antibody Registry ID:

Purified: AB_10673115
TC Supernatant: AB_10698179

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

95-110 kDa (varies with cell background due to phosphorylation)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.