Products: Primary Antibodies
PRODUCT OPTIONS
| Catalog # | Option | Volume | Concentration | Price* | |
|---|---|---|---|---|---|
| Catalog # 75-029 |
Option Purified |
Volume 100 µL |
Concentration 1 mg/mL |
Price $275.00 |
|
| Catalog # 73-029 |
Option TC Supernatant |
Volume 5 mL |
Concentration Lot dependent |
Price $275.00 |
Validation Images
![Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] probed with K28/86 (left) or N52A/42 (right) TC supe.](http://cdn.shopify.com/s/files/1/0014/8806/0475/products/validation-image-73-029-k28-86-wb-3.jpg?v=1549987714)
Overview
NeuroMab
Monoclonal
Mouse
IgG1
Human Mouse Rat Xenopus Zebrafish
IP WB
Fusion protein amino acids 77-299 of human PSD-95/SAP90
Rat: 100% identity (223/223 amino acids identical)
Mouse: 99% identity (221/223 amino acids identical)
65-80% identity with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102
Exhibits cross-reactivity against recombinant Chapsyn-110, SAP97 and SAP102
Disks large homolog 1 (Synapse-associated protein 97) (SAP-97) (SAP97) (hDlg) Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93) Disks large homolog 3 (Neuroendocrine-DLG) (Synapse-associated protein 102) (SAP-102) (SAP102) (XLMR) Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)
DLG1 DLG2 DLG3 KIAA1232 DLG4 PSD95
Purified: AB_10673115
TC Supernatant: AB_10698179
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
95-110 kDa(varies with cell background due to phosphorylation)
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
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- Bergeron SA, Carrier N, Li GH, Ahn S, Burgess HA.. (2015), 'Gsx1 expression defines neurons required for prepulse inhibition. .' Molecular Psychiatry. 10.1038/mp.2014.106. " "
- Murata Y, Constantine-Paton M.. (2013), 'Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2896-12.2013. " "
- Martin EA, Muralidhar S, Wang Z, Cervantes DC, Basu R, Taylor MR, Hunter J, Cutforth T, Wilke SA, Ghosh A, Williams ME.. (2015), 'The intellectual disability gene Kirrel3 regulates target-specific mossy fiber synapse development in the hippocampus. .' Elife. 10.7554/eLife.09395. "
- Krishnaswamy A#, Yamagata M#, Duan X, Hong YK, Sanes JR.. (2015), 'Sidekick 2 directs formation of a retinal circuit that detects differential motion..' Nature. 10.1038/nature14682. "
- Gopal SR, Chen DH, Chou SW, Zang J, Neuhauss SC, Stepanyan R, McDermott BM Jr, Alagramam KN.. (2015), 'Zebrafish Models for the Mechanosensory Hair Cell Dysfunction in Usher Syndrome 3 Reveal That Clarin-1 Is an Essential Hair Bundle Protein. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1096-15.2015. " "
- Dow E, Siletti K, Hudspeth AJ.. (2015), 'Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis. .' Genes & Development. 0.1101/gad.259838.115. " "
- Chassefeyre R, Martínez-Hernández J, Bertaso F, Bouquier N, Blot B, Laporte M, Fraboulet S, Couté Y, Devoy A, Isaacs AM, Pernet-Gallay K, Sadoul R, Fagni L, Goldberg Y.. (2015), 'Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0586-14.2015. " "
- Sheets L, Hagen MW, Nicolson T.. (2014), 'Characterization of Ribeye subunits in zebrafish hair cells reveals that exogenous Ribeye B-domain and CtBP1 localize to the basal ends of synaptic ribbons. .' PLoS One. 10.1371/journal.pone.0107256. " "
- Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS.. (2014), 'Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens. .' Current Biology. 0.1016/j.cub.2014.05.046. " "
- Easley-Neal C, Fierro J Jr, Buchanan J, Washbourne P.. (2013), 'Late recruitment of synapsin to nascent synapses is regulated by Cdk5. .' Cell Report. 10.1016/j.celrep.2013.03.031. " "
- Randlett O, MacDonald RB, Yoshimatsu T, Almeida AD, Suzuki SC, Wong RO, Harris WA.. (2013), 'Cellular requirements for building a retinal neuropil. .' Cell Report. 10.1016/j.celrep.2013.01.020. " "
- Sheets L, Kindt KS, Nicolson T.. (2012), 'Presynaptic CaV1.3 channels regulate synaptic ribbon size and are required for synaptic maintenance in sensory hair cells. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3005-12.2012. " "
- Liu W, Chen E, Zhao XW, Wan ZP, Gao YR, Davey A, Huang E, Zhang L, Crocetti J, Sandoval G, Joyce MG, Miceli C, Lukszo J, Aravind L, Swat W, Brzostowski J, Pierce SK.. (2012), 'The scaffolding protein synapse-associated protein 97 is required for enhanced signaling through isotype-switched IgG memory B cell receptors. .' Science Signaling. 10.1126/scisignal.2002820. " "
- Kietrys D, Galantino M.. (2011), 'On ""An obese body mass increases the adverse effects..."" Bauer LO, Wu Z, Wolfson LI. Phys Ther. 2011;91:1063-1071. .' Physical Therapy. 10.2522/ptj.2011.91.9.1424.1. " "
- Kiraly DD, Lemtiri-Chlieh F, Levine ES, Mains RE, Eipper BA.. (2011), 'Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3143-11.2011. " "
- Restituito S, Khatri L, Ninan I, Mathews PM, Liu X, Weinberg RJ, Ziff EB.. (2011), 'Synaptic autoregulation by metalloproteases and γ-secretase. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2513-11.2011. " "
- Chen X, Nelson CD, Li X, Winters CA, Azzam R, Sousa AA, Leapman RD, Gainer H, Sheng M, Reese TS.. (2011), 'PSD-95 is required to sustain the molecular organization of the postsynaptic density. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5968-10.2011. " "
- Sheets L, Trapani JG, Mo W, Obholzer N, Nicolson T.. (2011), 'Ribeye is required for presynaptic Ca(V)1.3a channel localization and afferent innervation of sensory hair cells. .' Development. 10.1242/dev.059451. " "
- Micheva KD, Busse B, Weiler NC, O'Rourke N, Smith SJ.. (2010), 'Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. .' Neuron. 10.1016/j.neuron.2010.09.024. "
- Sharma N, Deppmann CD, Harrington AW, St Hillaire C, Chen ZY, Lee FS, Ginty DD.. (2010), 'Long-distance control of synapse assembly by target-derived NGF..' Neuron. 10.1016/j.neuron.2010.07.018.
- Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L.. (2010), 'Visualizing the distribution of synapses from individual neurons in the mouse brain..' PLoS One. 10.1371/journal.pone.0011503. "
- Yamagata M, Sanes JR.. (2009), 'Synaptic localization and function of Sidekick recognition molecules require MAGI scaffolding proteins. .' Journal of Neuroscience. 10.1523/JNEUROSCI.6319-09.2010. "
- Davis EK, Zou Y, Ghosh A.. (2008), 'Wnts acting through canonical and noncanonical signaling pathways exert opposite effects on hippocampal synapse formation..' Neural Development. 10.1186/1749-8104-3-32. "
- Ross BS, Conroy WG.. (2008), 'Capabilities of neurexins in the chick ciliary ganglion. .' Developmental Neurobiology. 10.1002/dneu.20598. "