Products: Primary Antibodies

Anti-Pan-MAGUK (K28/86)

Download Full Datasheet

PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-029	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-029	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus Zebrafish

Applications:

IP WB

Immunogen:

Fusion protein amino acids 77-299 of human PSD-95/SAP90
Rat: 100% identity
Mouse=99% identity (221/223 amino acids)

Cross Reactivity:

Exhibits cross-reactivity against recombinant Chapsyn-110, SAP97 and SAP102

Protein Name(s):

Disks large homolog 1 (Synapse-associated protein 97) (SAP-97) (SAP97) (hDlg) Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93) Disks large homolog 3 (Neuroendocrine-DLG) (Synapse-associated protein 102) (SAP-102) (SAP102) (XLMR) Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Gene Name(s):

DLG1 DLG2 DLG3 KIAA1232 DLG4 PSD95

Antibody Registry ID:

Purified: AB_10673115
TC Supernatant: AB_10698179

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

95-110 kDa(varies with cell background due to phosphorylation)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Bergeron SA, Carrier N, Li GH, Ahn S, Burgess HA.. (2015), 'Gsx1 expression defines neurons required for prepulse inhibition. .' Molecular Psychiatry. 10.1038/mp.2014.106.
Murata Y, Constantine-Paton M.. (2013), 'Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2896-12.2013.
Martin EA, Muralidhar S, Wang Z, Cervantes DC, Basu R, Taylor MR, Hunter J, Cutforth T, Wilke SA, Ghosh A, Williams ME.. (2015), 'The intellectual disability gene Kirrel3 regulates target-specific mossy fiber synapse development in the hippocampus. .' Elife. 10.7554/eLife.09395.
Krishnaswamy A#, Yamagata M#, Duan X, Hong YK, Sanes JR.. (2015), 'Sidekick 2 directs formation of a retinal circuit that detects differential motion..' Nature. 10.1038/nature14682.
Gopal SR, Chen DH, Chou SW, Zang J, Neuhauss SC, Stepanyan R, McDermott BM Jr, Alagramam KN.. (2015), 'Zebrafish Models for the Mechanosensory Hair Cell Dysfunction in Usher Syndrome 3 Reveal That Clarin-1 Is an Essential Hair Bundle Protein. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1096-15.2015.
Dow E, Siletti K, Hudspeth AJ.. (2015), 'Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis. .' Genes & Development. 0.1101/gad.259838.115.
Chassefeyre R, Martínez-Hernández J, Bertaso F, Bouquier N, Blot B, Laporte M, Fraboulet S, Couté Y, Devoy A, Isaacs AM, Pernet-Gallay K, Sadoul R, Fagni L, Goldberg Y.. (2015), 'Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0586-14.2015.
Sheets L, Hagen MW, Nicolson T.. (2014), 'Characterization of Ribeye subunits in zebrafish hair cells reveals that exogenous Ribeye B-domain and CtBP1 localize to the basal ends of synaptic ribbons. .' PLoS One. 10.1371/journal.pone.0107256.
Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS.. (2014), 'Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens. .' Current Biology. 0.1016/j.cub.2014.05.046.
Easley-Neal C, Fierro J Jr, Buchanan J, Washbourne P.. (2013), 'Late recruitment of synapsin to nascent synapses is regulated by Cdk5. .' Cell Report. 10.1016/j.celrep.2013.03.031.
Randlett O, MacDonald RB, Yoshimatsu T, Almeida AD, Suzuki SC, Wong RO, Harris WA.. (2013), 'Cellular requirements for building a retinal neuropil. .' Cell Report. 10.1016/j.celrep.2013.01.020.
Sheets L, Kindt KS, Nicolson T.. (2012), 'Presynaptic CaV1.3 channels regulate synaptic ribbon size and are required for synaptic maintenance in sensory hair cells. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3005-12.2012.
Liu W, Chen E, Zhao XW, Wan ZP, Gao YR, Davey A, Huang E, Zhang L, Crocetti J, Sandoval G, Joyce MG, Miceli C, Lukszo J, Aravind L, Swat W, Brzostowski J, Pierce SK.. (2012), 'The scaffolding protein synapse-associated protein 97 is required for enhanced signaling through isotype-switched IgG memory B cell receptors. .' Science Signaling. 10.1126/scisignal.2002820.
Kietrys D, Galantino M.. (2011), 'On ""An obese body mass increases the adverse effects..."" Bauer LO, Wu Z, Wolfson LI. Phys Ther. 2011;91:1063-1071. .' Physical Therapy. 10.2522/ptj.2011.91.9.1424.1.
Kiraly DD, Lemtiri-Chlieh F, Levine ES, Mains RE, Eipper BA.. (2011), 'Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3143-11.2011.
Restituito S, Khatri L, Ninan I, Mathews PM, Liu X, Weinberg RJ, Ziff EB.. (2011), 'Synaptic autoregulation by metalloproteases and γ-secretase. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2513-11.2011.
Chen X, Nelson CD, Li X, Winters CA, Azzam R, Sousa AA, Leapman RD, Gainer H, Sheng M, Reese TS.. (2011), 'PSD-95 is required to sustain the molecular organization of the postsynaptic density. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5968-10.2011.
Sheets L, Trapani JG, Mo W, Obholzer N, Nicolson T.. (2011), 'Ribeye is required for presynaptic Ca(V)1.3a channel localization and afferent innervation of sensory hair cells. .' Development. 10.1242/dev.059451.
Micheva KD, Busse B, Weiler NC, O'Rourke N, Smith SJ.. (2010), 'Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. .' Neuron. 10.1016/j.neuron.2010.09.024.
Sharma N, Deppmann CD, Harrington AW, St Hillaire C, Chen ZY, Lee FS, Ginty DD.. (2010), 'Long-distance control of synapse assembly by target-derived NGF..' Neuron. 10.1016/j.neuron.2010.07.018.
Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L.. (2010), 'Visualizing the distribution of synapses from individual neurons in the mouse brain..' PLoS One. 10.1371/journal.pone.0011503.
Yamagata M, Sanes JR.. (2009), 'Synaptic localization and function of Sidekick recognition molecules require MAGI scaffolding proteins. .' Journal of Neuroscience. 10.1523/JNEUROSCI.6319-09.2010.
Davis EK, Zou Y, Ghosh A.. (2008), 'Wnts acting through canonical and noncanonical signaling pathways exert opposite effects on hippocampal synapse formation..' Neural Development. 10.1186/1749-8104-3-32.
Ross BS, Conroy WG.. (2008), 'Capabilities of neurexins in the chick ciliary ganglion. .' Developmental Neurobiology. 10.1002/dneu.20598.