Products: Primary Antibodies

Anti-Pan-Kvbeta potassium channel Antibody (K25/73)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-392	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-392	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

The ß subunits of voltage-gated potassium channels are members of the potassium channel, voltage-gated, shaker-related subfamily. Kvßs are cytoplasmic subunits that modulate the characteristics of the membrane spanning, channel-forming alpha-subunits. Beta subunits can promote the closure of channels or enhance channel activity depending on other channel proteins. There are three ß subunits (Kvß1, Kvß2, Kvß3) which are coded by three different genes. Additionally, there is alternative splicing of these three genes, further diversifying the functions and tissue expression of the family.

Immunogen:

Synthetic peptide amino acids 350-367 (EIDSILGNKPYSKKDYRS, C-terminus) of human Kvbeta2 (also known as Voltage-gated potassium channel subunit beta-2, K(+) channel subunit beta-2, Neuroimmune protein F5, KCNAB2, KCNA2B, KCNK2, Ckbeta2, I2rf5 and Kcnb3, accession number Q13303) Mouse: 100% identity (18/18 amino acids identical)
Rat: 100% identity (18/18 amino acids identical) 75% identity with Kvbeta1 and Kvbeta3

Cross Reactivity:

Reacts with Kvbeta1

Protein Name(s):

Voltage-gated potassium channel subunit beta-1 (EC 1.1.1.-) (K(+) channel subunit beta-1) (Kv-beta-1) Voltage-gated potassium channel subunit beta-3 (K(+) channel subunit beta-3) (Kv-beta-3) (RCK beta3) Voltage-gated potassium channel subunit beta-2 (EC 1.1.1.-) (K(+) channel subunit beta-2) (Kv-beta-2)

Gene Name(s):

Kcnab1 Kvb1 Kcnab3 Ckbeta3 Kcnab2 Ckbeta2 Kcnb3

Antibody Registry ID:

Purified: AB_2491089
TC Supernatant: AB_2336914

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

40 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.