A complex diluent to control background signal in biological samples.

Sample Diluent - Non-Mammalian-based is formulated to provide a complex yet protein-friendly environment for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA-format assays. Due to the finite binding capacity of plate-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument and to create a functional standard curve.

Sample Diluent - Non-Mammalian-based minimizes backgrounds and increases assay specificity. It has proven to be highly effective for routine dilution of mouse, porcine, bovine, or rabbit serum samples in antigen-down ELISAs. When testing serum and plasma samples, non-specific adsorption of sample IgG to the ELISA plate surface is a common cause of high background noise. In particular, the glycosylation pattern of porcine serum IgG tends to make porcine samples more ‘sticky’ than IgGs from other species, such as rabbit or mouse. Sample Diluent - Non-Mammalian-based is formulated to reduce this non-specific interaction so that porcine serum samples can be tested without needing a dilution factor beyond 1:100.

Sample Diluent - Non-Mammalian-based provides a non-mammalian protein-buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.