Mito Flow - Mitochondrial Membrane Potential Detection Kit

Name: Mito Flow - Mitochondrial Membrane Potential Detection Kit
Brand: ImmunoChemistry Technologies
SKU: Flo200-3
Price: 571.35 USD
Availability: InStock

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

Figure 1. Jurkat cells were stimulated with Staurosporine for 3 hours (B) or DMSO (A). Mito Flow reagent was added according to the protocol, incubated for 30 minutes and analyzed by flow cytometry: Ex:488nm Em: FL2 channel.

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Data Sheet Safety Data Sheet

SKU: Flo200-3

Size: 500 Tests

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$571.35

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Ships: 1-2 business days

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Scientific Background

The loss of mitochondrial membrane potential is a hallmark for apoptosis. The mitochondrial permeability transition is an important step in the induction of cellular apoptosis. During this process, the electrochemical gradient across the mitochondrial membrane collapses. The collapse is thought to occur through the formation of pores in the mitochondria by dimerized Bax or activated Bid, Bak, or Bad proteins. Activation of these pro-apoptotic proteins is accompanied by the release of cytochrome c into the cytoplasm.

The mitochondrial membrane potential detection kit assay utilizes cationic dye to visualize mitochondrial membrane potential. The reagent is a cell permeable cationic dye that has a strong fluorescent signal in the red region and exhibits low membrane potential independent (non specific) binding and toxicity. In healthy cells Mito Flow reagent is accumulated by the mitochondria in proportion to the DeltaPsi (membrane potential). In most cell lines, accumulation of the reagent in the mitochondria results in a higher fluorescence intensity. In apoptotic cells, where the mitochondrial membrane potential is compromised, the reagent does not accumulate in the mitochondria and these cells exhibit a lower fluorescence signal.

Please see protocol for instructions and useful hints and tips.

Method of Analysis

Flow Cytometer

Usage Statement

For research use only. Not for use in diagnostic procedures.

Country of Origin

United States

Storage

2-8°C

Shipping

Ships overnight (domestic), International Priority Shipping

Mito Flow – Mitochondrial Membrane Potential Detection Kit Protocol

Flo200-2: 100 Tests

Part # 4004: Vial of Mito Flow Dye x 1 (2 - 8 C)

Part # 3004: 10X Dilution Buffer x 1 (2 - 8 C)

Flo200-3: 500 Tests

Part # 4004: Vial of Mito Flow Dye x 5 (2 - 8 C)

Part # 3004: 10X Dilution Buffer x 5 (2 - 8 C)

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Mito Flow - Mitochondrial Membrane Potential Detection Kit

SKU: Flo200-3

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