Mito Flow - Mitochondrial membrane potential detection kit

Flo200-2 is an easy to use kit which measures mitochondrial membrane potential using flow cytometry. Useful for analysing both suspension and monolayer adherent cell lines.

SKU: Flo200-2

Size: 100 Tests
Sale price$279.00

The loss of mitochondrial membrane potential is a hallmark for apoptosis. The mitochondrial permeability transition is an important step in the induction of cellular apoptosis. During this process, the electrochemical gradient across the mitochondrial membrane collapses. The collapse is thought to occur through the formation of pores in the mitochondria by dimerized Bax or activated Bid, Bak, or Bad proteins. Activation of these pro-apoptotic proteins is accompanied by the release of cytochrome c into the cytoplasm.

The mitochondrial membrane potential detection kit assay utilizes cationic dye to visualize mitochondrial membrane potential. The reagent is a cell permeable cationic dye that has a strong fluorescent signal in the red region and exhibits low membrane potential independent (non specific) binding and toxicity. In healthy cells Mito Flow reagent is accumulated by the mitochondria in proportion to the DeltaPsi (membrane potential). In most cell lines, accumulation of the reagent in the mitochondria results in a higher fluorescence intensity. In apoptotic cells, where the mitochondrial membrane potential is compromised, the reagent does not accumulate in the mitochondria and these cells exhibit a lower fluorescence signal.

Please see protocol for instructions and useful hints and tips.

Flow Cytometer
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United States
Flo200-2: 100 Tests
  • Part # 4004: Vial of Mito Flow Dye x 1 (2 - 8 C)
  • Part # 3004: 10X Dilution Buffer x 1 (2 - 8 C)

  • Flo200-3: 500 Tests
  • Part # 4004: Vial of Mito Flow Dye x 5 (2 - 8 C)
  • Part # 3004: 10X Dilution Buffer x 5 (2 - 8 C)
  • Product Specific References

    PMID Publication
    31827709Zhao, B., et al. 2019. Metformin Suppresses Self-Renewal Ability and Tumorigenicity of Osteosarcoma Stem Cells via Reactive Oxygen Species-Mediated Apoptosis and Autophagy. Oxidative Medicine and Cellular Longevity, 9290728.
    34827081 Kaushik, N., et al. 2021. Blockade of Cellular Energy Metabolism through 6-Aminonicotinamide Reduces Proliferation of Non-Small Lung Cancer Cells by Inducing Endoplasmic Reticulum Stress. Biology, 1088.

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