Products: Primary Antibodies

Anti-Kv8.2 potassium channel Antibody (N448/88)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-435	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-435	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against membrane fractions from adult rat brain (RBM) and heart (RHM) and Kcnv2-S2 mouse wild-type (+/+) and higher-expressing transgenic (Tg/+) brains probed with N448/88 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Jennifer Kearney (Northwestern).$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Mouse

Applications:

ICC WB

Target Description:

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. Members of the Kv5-Kv11 families code for “silent subunits” that do not express as functional homomultimers. In heterologous expression systems, silent subunits can coassemble with Kv2 and Kv3 subunits and modulate the biophysical characteristics of the latter subunits (Yan L. et al., 2004).

Immunogen:

Fusion protein amino acids 1-163 (cytoplasmic N-terminus) of mouse Kv8.2 (also known as Potassium voltage-gated channel subfamily V member 2, Kcnv2 and rCG_63626, accession number Q8CFS6) Rat: 85% identity (138/163 amino acids identical)
Human: 70% identity (115/164 amino acids identical)

Protein Name(s):

Potassium voltage-gated channel subfamily V member 2 (Voltage-gated potassium channel subunit Kv8.2)

Gene Name(s):

Kcnv2

Antibody Registry ID:

Purified: AB_2651161
TC Supernatant: AB_2532091

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

65 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.