Products: Primary Antibodies

Anti-Kv7.1/KCNQ1 K+ channel (N37A/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-081	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-081	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 2-101 of human KCNQ1(Kv7.1, KvLQT1, accession number P51787)

Rat: 72% identity (71/98 amino acids identical)
Mouse: 63% identity (62/98 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Potassium voltage-gated channel subfamily KQT member 1 (IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1) (KQT-like 1) (Voltage-gated potassium channel subunit Kv7.1)

Gene Name(s):

KCNQ1 KCNA8 KCNA9 KVLQT1

Antibody Registry ID:

Purified: AB_2131682
TC Supernatant: AB_10675286

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Testai L, Barrese V, Soldovieri MV, Ambrosino P, Martelli A, Vinciguerra I, Miceli F, Greenwood IA, Curtis MJ, Breschi MC, Sisalli MJ, Scorziello A, Canduela MJ, Grandes P, Calderone V, Taglialatela M.. (2016), 'Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection. .' Cardiovascular Research. 10.1093/cvr/cvv281.
Salomonsson M, Brasen JC,, Braunstein TH, Hagelqvist P, Holstein-Rathlou NH,, Sorensen CM.. (2015), 'K(V)7.4 channels participate in the control of rodent renal vascular resting tone. .' Acta Physiologica. 10.1111/apha.12525.
Evseev AI, Semenov I, Archer CR, Medina JL, Dube PH, Shapiro MS, Brenner R.. (2013), 'Functional effects of KCNQ K(+) channels in airway smooth muscle. .' Frontiers in Physiology. 10.3389/fphys.2013.00277.
Sachse CC, Kim YH, Agsten M, Huth T, Alzheimer C, Kovacs DM, Kim DY.. (2013), 'BACE1 and presenilin/γ-secretase regulate proteolytic processing of KCNE1 and 2, auxiliary subunits of voltage-gated potassium channels. .' The FASEB Journal. 10.1096/fj.12-214056.
Krzystanek K, Rasmussen HB, Grunnet M, Staub O, Olesen SP, Abriel H, Jespersen T.. (2012), 'Deubiquitylating enzyme USP2 counteracts Nedd4-2-mediated downregulation of KCNQ1 potassium channels. .' Heart Rhythm. 10.1016/j.hrthm.2011.10.026.
Bajwa PJ, Lee JW, Straus DS, Lytle C.. (2009), 'Activation of PPARgamma by rosiglitazone attenuates intestinal Cl- secretion. .' Am The Journal of Physiology Gastrointest Liver Physiol.. 10.1152/ajpgi.90640.2008.