Human Mouse Rat
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Fusion protein amino acids 342-431 (cytoplasmic loop) of human GlyRAlpha3L (also known as Glycine receptor subunit alpha-3 and GLRA3, accession number O75311)
Mouse: 95% identity (86/90 amino acids identical)
Rat: 93% identity (84/90 amino acids identical)80% identity with GlyRAlpha3K (amino acids 358-372 or TEAFALEKFYRFSDM is L-specific)
~50% identity with GlyRAlpha1 and GlyRAlpha2
Cross-reacts with GlyRAlpha3K
Glycine receptor subunit alpha-3
TC Supernatant: AB_2491185
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.