Human Mouse Rat
ICC IHC WB
Human Glutamic Acid Decarboxylase (GAD-67), [EC 220.127.116.11] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter beta-amino butyric acid (GABA). It is also know as GAD-1.
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized through a 0.45 µm filter.
Glutamate decarboxylase 1 (EC 18.104.22.168) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)
GAD1 GAD GAD67
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Bigler RL, Kamande JW, Dumitru R, Niedringhaus M, Taylor AM. (2017), 'Messenger RNAs localized to distal projections of human stem cell derived neurons.' Nature Scientific Reports. 10.1038/s41598-017-00676-w.
- Nagendran T, Larsen RS, Bigler RL, Frost SB, Philpot BD, Nudo RJ, Taylor AM. (2017), 'Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling.' Natrue Communications. 10.1038/s41467-017-00652-y.