Products: Primary Antibodies

Anti-Glutamic Acid Decarboxylase-67 (GAD 67)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # GAD		Volume Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture.	Concentration 100 µg/ml	Price $399.00

Overview

Brand:

Aves Labs

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized through a 0.45 µm filter.

Protein Name(s):

Glutamate decarboxylase 1 (EC 4.1.1.15) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)

Gene Name(s):

GAD1 GAD GAD67

Antibody Registry ID:

Default Title: AB_2313545

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Expected Banding Pattern:

67 kDa

Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:500-1:1000

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Citations and References

Bigler RL, Kamande JW, Dumitru R, Niedringhaus M, Taylor AM. (2017), 'Messenger RNAs localized to distal projections of human stem cell derived neurons.' Nature Scientific Reports. 10.1038/s41598-017-00676-w.
Nagendran T, Larsen RS, Bigler RL, Frost SB, Philpot BD, Nudo RJ, Taylor AM. (2017), 'Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling.' Natrue Communications. 10.1038/s41467-017-00652-y.