Products: Primary Antibodies
PRODUCT OPTIONS
| Catalog # | Option | Volume | Concentration | Price* | |
|---|---|---|---|---|---|
| Catalog # GAD |
Volume Regular vials contain 1000 µL of this antibody mixture; Sampler vials contain 200 µL of this mixture. |
Concentration 100 µg/ml |
Price $399.00 |
Validation Images
Overview
Aves Labs
Polyclonal
Chicken
Human Mouse Rat
ICC IHC WB
Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized through a 0.45 µm filter.
Glutamate decarboxylase 1 (EC 4.1.1.15) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)
GAD1 GAD GAD67
Default Title: AB_2313545
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
67 kDa
1:1000-1:2000
1:500-1:1000
Quality Control
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Citations and References
- Bigler RL, Kamande JW, Dumitru R, Niedringhaus M, Taylor AM. (2017), 'Messenger RNAs localized to distal projections of human stem cell derived neurons.' Nature Scientific Reports. 10.1038/s41598-017-00676-w.
- Nagendran T, Larsen RS, Bigler RL, Frost SB, Philpot BD, Nudo RJ, Taylor AM. (2017), 'Distal axotomy enhances retrograde presynaptic excitability onto injured pyramidal neurons via trans-synaptic signaling.' Natrue Communications. 10.1038/s41467-017-00652-y.