Products: Primary Antibodies

Anti-GluA1/GluR1 glutamate receptor Antibody (N355/1)

from NeuroMab

PRODUCT OPTIONS

★ Trial Size Available ★

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-327	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $325.00
Brand	Catalog # 75-327-020	Option Purified Trial-Size	Volume 20 µL	Concentration 1mg/mL	Price $109.00
Brand	Catalog # 73-327	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $325.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Glutamate receptor 1 or GluA1/GluR1 glutamate receptor is a member of the glutamate-gated ion channel family. GluR1 funcitons as an excitatory neurotransmitter at many synapses in the central nervous system and is widely expressed in brain. Mutations in the GluR1 gene are associated with cerebral cortical dysplasia, Status Epilepticus, and depression.

Immunogen:

Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 (also known as Glutamate receptor 1, Ionotropic AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, GLUH1 and Gria1, accession number P19490) Mouse: 99% identity (388/389 amino acids identical)
Human: 97% identity (379/389 amino acids identical)
100% identity between Flip and Flop isoforms
>50% identity with GluA2/GluR2, GluA3/GluR3 and GluA4/GluR4

Cross Reactivity:

Does not cross-react with GluR2

Protein Name(s):

Glutamate receptor 1 (GluR-1) (AMPA-selective glutamate receptor 1) (GluR-A) (GluR-K1) (Glutamate receptor ionotropic, AMPA 1) (GluA1)

Gene Name(s):

Gria1 Glur1

Antibody Registry ID:

Purified: AB_2315840
Purified Trial-Size: AB_2315840
TC Supernatant: AB_2315839

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY. (2016), 'Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts..' Cell.. 10.1016/j.cell.2016.07.041.
Vingtdeux V, Chang EH, Frattini SA, Zhao H, Chandakkar P, Adrien L, Strohl JJ, Gibson EL, Ohmoto M, Matsumoto I, Huerta PT, Marambaud P. (2016), 'CALHM1 deficiency impairs cerebral neuron activity and memory flexibility in mice..' Sci Rep.. 10.1038/srep24250.
Moraga-Amaro R, González H, Ugalde V, Donoso-Ramos JP, Quintana-Donoso D, Lara M, Morales B, Rojas P, Pacheco R, Stehberg J. (2016), 'Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory..' Neuropharmacology.. 10.1016/j.neuropharm.2015.12.018.
Bellizzi MJ, Geathers JS, Allan KC, Gelbard HA. (2016), 'Platelet-Activating Factor Receptors Mediate Excitatory Postsynaptic Hippocampal Injury in Experimental Autoimmune Encephalomyelitis..' J Neurosci.. 10.1523/JNEUROSCI.1171-15.2016.
Lv JM, Guo XM, Chen B, Lei Q, Pan YJ, Yang Q. (2016), 'The Noncompetitive AMPAR Antagonist Perampanel Abrogates Brain Endothelial Cell Permeability in Response to Ischemia: Involvement of Claudin-5..' Cell Mol Neurobiol.. 10.1007/s10571-015-0257-8.
Tempia F, Hoxha E, Negro G, Alshammari MA, Alshammari TK, Panova-Elektronova N, Laezza F. (2015), 'Parallel fiber to Purkinje cell synaptic impairment in a mouse model of spinocerebellar ataxia type 27..' Front Cell Neurosci.. 10.3389/fncel.2015.00205.
Varea O, Martin-de-Saavedra MD, Kopeikina KJ, Schürmann B, Fleming HJ, Fawcett-Patel JM, Bach A, Jang S, Peles E, Kim E, Penzes P. (2015), 'Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons..' Proc Natl Acad Sci U S A.. 10.1073/pnas.1423205112.
Cristino L, Luongo L, Squillace M, Paolone G, Mango D, Piccinin S, Zianni E, Imperatore R, Iannotta M, Longo F, Errico F, Vescovi AL, Morari M, Maione S, Gardoni F, Nisticò R, Usiello A. (2015), 'd-Aspartate oxidase influences glutamatergic system homeostasis in mammalian brain..' Neurobiol Aging.. 10.1016/j.neurobiolaging.2015.02.003.
de Andrade GB, Kunzelman L, Merrill MM, Fuerst PG. (2014), 'Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina..' Mol Vis.. .
Parkhurst CN, Yang G, Ninan I, Savas JN, Yates JR rd, Lafaille JJ, Hempstead BL, Littman DR, Gan WB. (2013), 'Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor..' Cell.. 10.1016/j.cell.2013.11.030.
Luo P, Yang Y, Liu W, Rao W, Bian H, Li X, Chen T, Liu M, Zhao Y, Dai S, Yan X, Fei Z. (2013), 'Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons..' FEBS J.. 10.1111/febs.12531.