Products: Primary Antibodies

Anti-GluA1/GluR1 glutamate receptor (N355/1)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-327	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $319.00
Catalog # 73-327	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $319.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1 (also known as Glutamate receptor 1, Ionotropic AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, GLUH1 and Gria1, accession number P19490) Mouse: 99% identity (388/389 amino acids identical) Human: 97% identity (379/389 amino acids identical) 100% identity between Flip and Flop isoforms >50% identity with GluA2/GluR2, GluA3/GluR3 and GluA4/GluR4

Cross Reactivity:

Does not cross-react with GluR2

Protein Name(s):

Glutamate receptor 1 (GluR-1) (AMPA-selective glutamate receptor 1) (GluR-A) (GluR-K1) (Glutamate receptor ionotropic, AMPA 1) (GluA1)

Gene Name(s):

Gria1 Glur1

Antibody Registry ID:

Purified: AB_2315840
TC Supernatant: AB_2315839

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Loh KH1, Stawski PS1, Draycott AS1, Udeshi ND2, Lehrman EK3, Wilton DK3, Svinkina T2, Deerinck TJ4, Ellisman MH4, Stevens B3, Carr SA2, Ting AY5.. (2016), 'Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts..' Cell.. 10.1016/j.cell.2016.07.041.
Vingtdeux V1, Chang EH2, Frattini SA2, Zhao H1, Chandakkar P1, Adrien L1, Strohl JJ2, Gibson EL2, Ohmoto M3, Matsumoto I3, Huerta PT2,4, Marambaud P1.. (2016), 'CALHM1 deficiency impairs cerebral neuron activity and memory flexibility in mice..' Sci Rep.. 10.1038/srep24250.
Moraga-Amaro R1, González H2, Ugalde V2, Donoso-Ramos JP1, Quintana-Donoso D1, Lara M3, Morales B3, Rojas P3, Pacheco R4, Stehberg J5.. (2016), 'Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory..' Neuropharmacology.. 10.1016/j.neuropharm.2015.12.018.
Bellizzi MJ1, Geathers JS2, Allan KC2, Gelbard HA3.. (2016), 'Platelet-Activating Factor Receptors Mediate Excitatory Postsynaptic Hippocampal Injury in Experimental Autoimmune Encephalomyelitis..' J Neurosci.. 10.1523/JNEUROSCI.1171-15.2016.
Lv JM1, Guo XM1, Chen B2, Lei Q1, Pan YJ1, Yang Q3.. (2016), 'The Noncompetitive AMPAR Antagonist Perampanel Abrogates Brain Endothelial Cell Permeability in Response to Ischemia: Involvement of Claudin-5..' Cell Mol Neurobiol.. 10.1007/s10571-015-0257-8.
Tempia F1, Hoxha E2, Negro G3, Alshammari MA4, Alshammari TK4, Panova-Elektronova N5, Laezza F6.. (2015), 'Parallel fiber to Purkinje cell synaptic impairment in a mouse model of spinocerebellar ataxia type 27..' Front Cell Neurosci. . 10.3389/fncel.2015.00205.
Varea O1, Martin-de-Saavedra MD1, Kopeikina KJ1, Schürmann B1, Fleming HJ1, Fawcett-Patel JM1, Bach A1, Jang S2, Peles E3, Kim E4, Penzes P5.. (2015), 'Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons..' Proc Natl Acad Sci U S A.. 10.1073/pnas.1423205112.
Cristino L1, Luongo L2, Squillace M3, Paolone G4, Mango D5, Piccinin S5, Zianni E6, Imperatore R1, Iannotta M2, Longo F4, Errico F7, Vescovi AL8, Morari M4, Maione S2, Gardoni F6, Nisticò R9, Usiello A10.. (2015), 'd-Aspartate oxidase influences glutamatergic system homeostasis in mammalian brain..' Neurobiol Aging. . 10.1016/j.neurobiolaging.2015.02.003.
de Andrade GB1, Kunzelman L2, Merrill MM3, Fuerst PG4.. (2014), 'Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina..' Mol Vis.. .
Parkhurst CN1, Yang G2, Ninan I3, Savas JN4, Yates JR 3rd4, Lafaille JJ5, Hempstead BL6, Littman DR7, Gan WB8.. (2013), 'Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor..' Cell.. 10.1016/j.cell.2013.11.030.
Luo P1, Yang Y, Liu W, Rao W, Bian H, Li X, Chen T, Liu M, Zhao Y, Dai S, Yan X, Fei Z.. (2013), 'Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons..' FEBS J.. 10.1111/febs.12531.