Products: Primary Antibodies

Anti-Cav1.2 Ca2+ channel (N263/31)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-257		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$275.00
Catalog #
73-257		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Immunogen:

Fusion protein amino acids 808-874 (cytoplasmic loop between repeat II and III) of rat Cav1.2 (also known as Voltage-dependent L-type calcium channel subunit alpha-1C, CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1, Brain class C and MELC-CC, accession number Q5S007)

Mouse: 100% identity (67/67 amino acids identical)
Human: 86% identity (58/67 amino acids identical) <50% identity with other Cav1 channels

Cross Reactivity:

Does not cross-react with Cav1.3

Protein Name(s):

Voltage-dependent L-type calcium channel subunit alpha-1C (Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle) (Rat brain class C) (RBC) (Voltage-gated calcium channel subunit alpha Cav1.2)

Gene Name(s):

Cacna1c Cach2 Cacn2 Cacnl1a1 Cchl1a1

Antibody Registry ID:

Purified: AB_11000167
TC Supernatant: AB_11001554

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

240 kDa

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References