Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody

Name: Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody
Brand: PhosphoSolutions
SKU: 2001-TFAM
Price: 375.0 USD
Availability: InStock

3 Citations

Our Anti-TFAM (Transcription Factor A, mitochondrial) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat TFAM (Transcription Factor A, motochondrial) and is neat serum. It is great for use in WB.

Western blot of 143B cells showing specific immunolabeling human TFAM (top row). Knockout validation is demonstrated in lane 2 where cells transfected with altered TFAM via GeneSwap show no immunolabeling. Image from publication CC-BY-4.0. PMID: 35883613

Western blot of rat kidney lysate showing specific immunolabeling of the ~24 kDa TFAM protein.

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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Data Sheet

KO Validated

Trial Size

SKU: 2001-TFAM

Volume: 100 µL

Variant

Price:

$375.00

Quantity:

Product Details

Target

TFAM (Transcription Factor A, motochondrial)

Target Description

Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

TFAM

Molecular Weight

24 kDa

Antigen

Native recombinant mouse TFAM protein with c-terminal 6-his tag

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492259

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat Serum

Buffer

Neat Serum

Dilution Ranges

WB: 1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~24 kDa TFAM protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Transcription factor 6-like 1 antibody, Mitochondrial transcription factor 1 antibody, mitochondrial transcription factor A antibody, MtTF1 antibody, mtTFA antibody, TCF 6 antibody, TCF-6 antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, TFAM antibody, TFAM_HUMAN antibody, Transcription factor 6 antibody, Transcription factor 6 like 2 (mitochondrial transcription factor) antibody, Transcription factor 6 like 2 antibody, Transcription factor 6-like 2 antibody, transcription factor 6-like 3 antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial precursor antibody

UniProt Details

UniProt (Immunogen Species): P40630

NCBI Gene ID

21780

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
37298383	not listed	Kozhukhar, N, et al. 2023. The C-Terminal Tail of Mitochondrial Transcription Factor A Is Dispensable for Mitochondrial DNA Replication and Transcription In Situ. International Journal of Molecular Sciences, 9430.
35883613	not listed	Kozhukhar, N., et al. 2022. A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication. Cells, 2168.
35487893	1:1000	Sidarala, V., et al. 2022. Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis. Nature Communications, 13(1), pp.1-16.

Bulk Order Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody

Product Specific Protocols

Western Blot Protocol: Download

General References

Hayashi, Y., Yoshida, M., Yamato, M., Ide, T., Wu, Z., Ochi-Shindou, M., Kanki, T., Kang, D., Sunagawa, K., Tsutsui, H. and Nakanishi, H., 2008. Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice. Journal of Neuroscience, 28(34), pp.8624-8634. PMID: 18716221
Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T. and Abe, K., 2012. Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice. Journal of Neuroscience Research, 90(6), pp.1200-1208. PMID: 22354563

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Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody

SKU: 2001-TFAM

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody

Product Specific Protocols

General References

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