Anti-RNA-binding Protein Musashi homolog 1 Antibody (3F2)

Name: Anti-RNA-binding Protein Musashi homolog 1 Antibody
Brand: Biosensis
SKU: M-1720-100
Price: 431.67 USD
Availability: InStock

Our Anti-RNA-binding protein Musashi homolog 1 mouse monoclonal primary antibody detects human RNA-binding protein Musashi homolog 1 , and is IgG. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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SKU: M-1720-100

Volume/Size: 100 µg

Variant

Price:

$431.67

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Product Details

Target

RNA-binding protein Musashi homolog 1

Scientific Background

Musashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.

Format

IgG

Clonality

Monoclonal

Clone

3F2

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Immunogen

Full length recombinant human Musashi 1 protein (1-87) with a GST tag.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for up to six months. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified immunoglobulin

Buffer

Lyophilized from PBS pH 7.4.

Dilution Ranges

IHC: 3-10 µg/mL
ICC: 3-10 µg/mL

Application Details

The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125ºC for 4 minutes then at 90ºC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes.

Conjugate

Unconjugated

Specificity

Specificity has been confirmed by WB and direct ELISA against the antigen.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

RNA-binding protein Musashi homolog 1; Musashi1; MSI1

UniProt ID

O43347

Shipping

25°C (ambient)

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Anti-RNA-binding Protein Musashi homolog 1 Antibody (3F2)

SKU: M-1720-100

Product Details

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