Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

Name: Anti-PSD-95 MAGUK Scaffold Protein Antibody
Brand: NeuroMab
SKU: 75-348
Price: 361.0 USD
Availability: InStock

from NeuroMab Datasheet KO Validated

3 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

K28/74

Applications

AT, ICC, IHC, WB

Host Species

Mouse

Format

Purified

Gene Name

DLG4 PSD95

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Go to Full Product Details

immunofluorescence of human frontal cortex with K28/74 (green), rabbit Synapsin-1 (red) and DAPI (blue). Twelve images from 100 nm sections (left) were volume-reconstructed via array tomography (31.5 x 34 x 1.2 mm, right). Image courtesy of Nancy O’Rourke, Nafisa Ghori and Stephen Smith (Stanford).

immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD- 95 or PSD-93 knockout (KO) mice probed with K28/74 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).

adult rat brain membrane immunoblot

Volume: 100 µL

Variant

Price:

$361

Quantity:

Bulk Order Anti-PSD-95 MAGUK Scaffold Protein Antibody

Product Details

Target

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Target Description

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K28/74

Isotype

IgG1

Applications

AT, ICC, IHC, WB

Host Species

Mouse

Gene Name

DLG4 PSD95

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Antigen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (also known as Postsynaptic density protein 95, Disks large homolog 4, Synapseassociated protein 90, DLG4, Dlgh4 and SAP-90, accession number P78352) Rat: 100% identity (223/223 amino acids identical) Mouse: 99% identity (221/223 amino acids identical) 65-80% identity with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315909

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Does not cross-react with DLG1/SAP97

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

UniProt Details

UniProt (Human): Q15334
UniProt (Immunogen Species): P78352

Howell MD, Bailey LA, Cozart MA, Gannon BM, Gottschall PE. (2015), 'Hippocampal administration of chondroitinase ABC increases plaque-adjacent synaptic marker and diminishes amyloid burden in aged APPswe/PS1dE9 mice..' Acta Neuropathol Commun. 10.1186/s40478-015-0233-z.
Puthussery T, Percival KA, Venkataramani S, Gayet-Primo J, Grünert U, Taylor WR. (2014), 'Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina..' J Neurosci. 10.1523/JNEUROSCI.4855-13.2014.
Karayannis T, Au E, Patel JC, Kruglikov I, Markx S, Delorme R, Héron D, Salomon D, Glessner J, Restituito S, Gordon A, Rodriguez-Murillo L, Roy NC, Gogos JA, Rudy B, Rice ME, Karayiorgou M, Hakonarson H, Keren B, Huguet G, Bourgeron T, Hoeffer C, Tsien RW, Peles E, Fishell G. (2014), 'Cntnap4 differentially contributes to GABAergic and dopaminergic synaptic transmission..' Nature. 10.1038/nature13248.

Recover password

Create my account

Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

Anti-PSD-95 MAGUK Scaffold Protein Antibody
SKU: 75-348

Bulk Order Anti-PSD-95 MAGUK Scaffold Protein Antibody

Product Details

Related Products

Recently Viewed

Anti-PSD-95 MAGUK Scaffold Protein Antibody (K28/74)

Anti-PSD-95 MAGUK Scaffold Protein Antibody SKU: 75-348

Bulk Order Anti-PSD-95 MAGUK Scaffold Protein Antibody

Product Details

Anti-PSD-95 MAGUK Scaffold Protein Antibody
SKU: 75-348