Anti-Prostatic Acid Phosphatase Antibody

Name: Anti-Prostatic Acid Phosphatase Antibody
Brand: Aves Labs
SKU: PAP
Price: 295.0 USD
Availability: InStock

9 Citations

Recombinant mouse PAP protein was expressed in bacteria. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been validated with human, mouse and rat tissues.

Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.

Adult mouse spinal cord was fixed in 4% paraformaldehyde, paraffin-embedded and sectioned, and then sections were stained for PAP immunoreactivity (1:500 dilution). Adjacent sections were co-stained for IB4 and Calcitonin Gene-Related Protein (CGRP) (other sensory neuronal markers). Photomicrograph by Dr. Mark Zilka, University of North Carolina.

Adult mouse DRG was fixed in 4% paraformaldehyde, cryostat-sectioned, and then stained for PAP immunoreactivity (1:500 dilution), showing immunoreactive material in primary sensory neurons. Photomicrograph by Dr. Mark Zilka, Univ. of North Carolina.

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Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Chicken

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Data Sheet

SKU: PAP

Volume: 200 µL

Variant

Price:

$295.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Prostatic Acid Phosphatase (PAP)

Scientific Background

Mouse Prostatic Acid Phosphatase (PAP) is a 43,698 dalton protein (381 amino acids; NCBI accession number AAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.

Format

Mixture of IgY fraction and affinity-purified antibodies

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

IHC, WB

Host Species

Chicken

Gene Name

ACPP

Molecular Weight

45 kDa

Immunogen

Recombinant mouse PAP expressed in Escherichia coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313557

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with recombinant mouse Prostatic Acid Phosphatase protein. After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared ("anti-PAP IgY fraction"). Some of this antibody was further purified using an agarose matrix to which the PAP protein was covalently attached ("Affinity-purified anti-PAP"). The final preparation in the accompanying vial contains 10 mg/mL of the "anti-PAP IgY fraction" supplemented with 20 µg/mL of the "affinity-purified anti-PAP" plus 50% (v/v) glycerol (to prevent freezing at –20˚C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 ul aliquots prepared.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000

Quality Control

Antibodies were analyzed using immunohistochemistry with tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAPpositive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Aves Cat. #F-1004) were used at a concentration of 1:500.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Prostatic acid phosphatase (PAP) (EC 3.1.3.2) (5'-nucleotidase) (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (Thiamine monophosphatase) (TMPase) [Cleaved into: PAPf39]

UniProt ID

P15309

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30578250		Lin, C.L., et al. 2018. Treatment with methyl-b-cyclodextrin prevents mechanical allodynia in resiniferatoxin neuropathy in a mouse model. Biology Open, bio039511.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
31291586		Boyle, K.A., et al. 2019. Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia.. Cell Reports, 526-540.
29672450	11:00	Kan, H.W., et al. 2018. Downregulation of adenosine and adenosine A1 receptor contributes to neuropathic pain in resiniferatoxin neuropathy. Pain, 1580-1591.
29022879		Olson, W., et al. 2017. Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors. eLife, e29507.
27306411	11:00	Wu, C.H., et al. 2016. NGF-trkA signaling modulates the analgesic effects of prostatic acid phosphatase in resiniferatoxin-induced neuropathy. Molecular Pain, .
20084276	1:4000	Taylor-Blake, B., et al. 2010. Prostatic acid phosphatase is expressed in peptidergic and nonpeptidergic nociceptive neurons of mice and rats. PLoS One, e8674.

Immunohistochemistry: Rat
PMID	Dilution	Publication
29943954	1:100	Nishida, K., et al. 2018. ATP metabolizing enzymes ENPP1, 2 and 3 are localized in sensory neurons of rat dorsal root ganglion. The European Journal of Histochemistry, 2877.
24284839	1:2000	Chen, L., et al. 2013. Antinociceptive effect of prostatic acid phosphatase in a rat model of cancer-induced bone pain. Pain Physician, 533-546.

Western Blot: Mouse
PMID	Dilution	Publication
20084276	1:40,000	Taylor-Blake, B., et al. 2010. Prostatic acid phosphatase is expressed in peptidergic and nonpeptidergic nociceptive neurons of mice and rats. PLoS One, e8674.

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Anti-Prostatic Acid Phosphatase Antibody

SKU: PAP

Product Details

Product Specific References for Applications and Species

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