Our Anti-Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects hamster, mouse and rat Potassium Channel, Voltage-Gated, Kv3.1 Subunit (Ser503) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Immunostaining of Chinese Hamster Overy (CHO) cells stably transfected with KV3.1b gene with the phospho-Ser503 Kv3.1 subunit antibody (cat. p1550-503, green, 1:400). The image on the right shows cells that have been treated with the protein kinase C activator PMA (500nM) while the control cells are on the left.
Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995; Pongs, 1999; Blaine and Ribera, 1998; Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999). Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ICC, IHC, WB
Rabbit
KCNC1
100 kDa
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser503 of the rat voltage-gated potassium channel Kv3.1, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
AB_2492212
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:400-1:1000
ICC: 1:400
Unconjugated
Specific for endogenous levels of the ~100 kDa Kv3.1 voltage-gated potassium channel protein phosphorylated at Ser503.
Phosphorylated
Ser503
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.
Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.
Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.
Song, P., et al. 2006. Modulation of Kv3. 1b potassium channel phosphorylation in auditory neurons by conventional and novel protein kinase C isozymes. Journal of Biological Chemistry, 281(22), pp.15582-15591.
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