Primary Antibody
Human, Mouse, Rat
N23B/49
ICC, IHC, IP, WB
Mouse
Purified by Protein A chromatography
Shank1 Shank2 Cortbp1 Kiaa1022 PROSAP1 Shank3 Kiaa1650 Prosap2 PSAP2
160 kDa
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-089-n23b-49-wb-2_{width}x.jpg?v=1626904240)
Cultured rat hippocampal neuron immunofluorescence: N23B/49 (Pan-Shank) = red, rabbit anti-PSD-95 = green, merge = yellow (bottom right). Image courtesy of Dr. Albert Hung, Picower Institute, MIT.
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.antibodiesinc.com/cdn/shop/products/validation-image-73-089-n23b-49-wb-2_1024x.jpg?v=1626904240)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- immunogold Labeling: Mouse | Rat
- Immunohistochemistry: Human | Mouse
- Probe QMI: Mouse
- Western Blot: Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 22922712 | 1:400 | Ricciardi,S., et al. 2012. CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons. Nature cell biology, 911-923. |
| 21926970 | 1:400 | Fogel, A., et al. 2011. Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation. The EMBO journal, 4728-4738. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 21813652 | not listed | Yoon, S., et al. 2020. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron, 506-521. |
| 27986928 | 1:500 | Lee, S.J., et al. 2017. Presynaptic Neuronal Pentraxin Receptor Organizes Excitatory and Inhibitory Synapses. The Journal of neuroscience : the official journal of the Society for Neuroscience, 1062-1080. |
| 24712994 | 1:100 | Lussier, M., et al. 2014. Casein kinase 2 phosphorylates GluA1 and regulates its surface expression. The European journal of neuroscience, 1148-1158. |
| 23719805 | 1:400 | Hoy, J., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. The Journal of neuroscience : the official journal of the Society for Neuroscience, 9364-9384. |
| 23209303 | 1:500 | Cheadle, L., et al. 2012. The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. The Journal of cell biology, 985-1001. |
| 21123580 | not listed | Malinverno, M., et al. 2010. Synaptic localization and activity of ADAM10 regulate excitatory synapses through N-cadherin cleavage. The Journal of neuroscience : the official journal of the Society for Neuroscience, 16343-16655. |
| 20696380 | 2ug/ml | Sharma, N., et al. 2010. Long-distance control of synapse assembly by target-derived NGF. Neuron, 422-434. |
| 20660256 | not listed | Moreau, M., et al. 2010. The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function. The Journal of neuroscience : the official journal of the Society for Neuroscience, 9738-9752. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36847487 | not listed | Song, J.M., et al. 2023. Deneddylating Enzyme SENP8 Regulates Neuronal Development. Journal of Neurochemistry, . |
| 35966009 | 6.25ug/ml | Dean, C.A., et al. 2022. Regulation of NMDA Receptor Signaling at Single Synapses by Human Anti-NMDA Receptor Antibodies. Frontiers in molecular neuroscience, 940005. |
| 29429936 | not listed | Matt, L., et al. 2018. α-Actinin Anchors PSD-95 at Postsynaptic Sites. Neuron, 1094-1109. |
| 27307232 | not listed | Yokoi ,N., et al. 2016. Identification of PSD-95 Depalmitoylating Enzymes. The Journal of neuroscience : the official journal of the Society for Neuroscience, 6431-6444. |
| 26675527 | not listed | Lee, N., et al. 2016. Hexa (ethylene glycol) derivative of benzothiazole aniline promotes dendritic spine formation through the RasGRF1-Ras dependent pathway. Biochimica et biophysica acta, 284-295. |
| 26547831 | 1:400 | MacGillavry, H., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. The European journal of neuroscience, 179-193. |
| 26679993 | 1:200 | Stanic, J., et al. 2015. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nature Communications, 10181. |
| 22328515 | not listed | Gardoni, F., et al. 2012. The neuropeptide PACAP38 induces dendritic spine remodeling through ADAM10-N-cadherin signaling pathway. The Journal of cell science, 1401-1406. |
| 21795692 | not listed | Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34839-34850. |
| 20338226 | 1:100 | Westerholz, S., et al. 2010. Regulation of early spontaneous network activity and GABAergic neurons development by thyroid hormone. Neuroscience, 573-589. |
| 20220006 | not listed | Perez de Arce, K., et al. 2010. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience, 3728-3738. |
| 20164337 | not listed | Zibetti, C., et al. 2010. Alternative splicing of the histone demethylase LSD1/KDM1 contributes to the modulation of neurite morphogenesis in the mammalian nervous system. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2521-2532. |
| 20118925 | 1:200 | Suh, Y.H., et al. 2010. A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking. Nature neuroscience, 338-343. |
| ImmunoGold Labeling: Mouse | ||
| PMID | Dilution | Publication |
| 27144302 | 1:250 | Tao-Cheng, J.H., et al. 2016. Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses. PLoS one, e0153979. |
| ImmunoGold Labeling: Rat | ||
| PMID | Dilution | Publication |
| 32238193 | 1:200 | Tao-Cheng, J.H., et al. 2020. Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons. Molecular brain, 53. |
| 29284046 | not listed | Dosemeci, A., et al. 2017. IRSp53 accumulates at the postsynaptic density under excitatory conditions. PLoS one, e0190250. |
| 25775468 | 1:250 | Tao-Cheng, J.H., et al. 2015. Differential distribution of Shank and GKAP at the postsynaptic density. PLoS one, e0118750. |
| 24952157 | not listed | Tao-Cheng, J.H., et al. 2014. NMDA-induced accumulation of Shank at the postsynaptic density is mediated by CaMKII. Biochemical and biophysical research communications, 808-811. |
| 20347015 | 1:200, 1:1000 | Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 11-17. |
| 20118925 | 1:200 | Suh, Y.H., et al. 2010. A neuronal role for SNAP-23 in postsynaptic glutamate receptor trafficking. Nature neuroscience, 338-343. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 20573181 | 1:1000 | Pham, E., et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. The FEBS journal, 3051-3067. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 25524173 | not listed | Serrano, F., et al. 2014. Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice. Molecular neurodegeneration, 61. |
| 25352748 | 1:400 | Belem de Andrade, G., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. Molecular vision, 1422-1433. |
| 20696380 | 2ug/ml | Sharma, N., et al. 2010. Long-distance control of synapse assembly by target-derived NGF. Neuron, 422-434. |
| 20660256 | not listed | Moreau, M., et al. 2010. The planar polarity protein Scribble1 is essential for neuronal plasticity and brain function. The Journal of neuroscience : the official journal of the Society for Neuroscience, 9738-9752. |
| 20573181 | 1:1000 | Pham, E., et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. The FEBS journal, 3051-3067. |
| Probe QMI: Mouse | ||
| PMID | Dilution | Publication |
| 34852231 | not listed | Lautz, J., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell reports, 110076. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 20980075 | not listed | Marcello, E., et al. 2012. SAP97-mediated local trafficking is altered in Alzheimer disease patients' hippocampus. Neurobiology of aging, 422.e1-10. |
| 20573181 | 1:1000 | Pham, E., et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. The FEBS journal, 3051-3067. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35966008 | not listed | Yoo, T., et al. 2022. A Deficiency of the Psychiatric Risk Gene DLG2/PSD-93 Causes Excitatory Synaptic Deficits in the Dorsolateral Striatum. Frontiers in molecular neuroscience, 938590. |
| 35662394 | 1:1000 | Dhume, S.H., et al. 2022. Distinct but overlapping roles of LRRTM1 and LRRTM2 in developing and mature hippocampal circuits. eLife, e64742. |
| 35368073 | 1:10 (supe) | Wang, T., et al. 2022. Sapap4 deficiency leads to postsynaptic defects and abnormal behaviors relevant to hyperkinetic neuropsychiatric disorder in mice. Cerebral cortex (New York, N.Y. : 1991), bhac123. |
| 31985401 | 1:1000 | Sclip, A., et al. 2020. LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses. eLife, e53406. |
| 21813652 | not listed | Yoon, S., et al. 2020. Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development. Neuron, 506-521. |
| 27663742 | 1:1000 | Sawicka, K., et al. 2016. Elevated ERK/p90 ribosomal S6 kinase activity underlies audiogenic seizure susceptibility in fragile X mice. Proceedings of the National Academy of Sciences of the United States of America, E6290-E6297. |
| 25653350 | not listed | Zhang, N., et al. 2015. S-SCAM, a rare copy number variation gene, induces schizophrenia-related endophenotypes in transgenic mouse model. The Journal of neuroscience : the official journal of the Society for Neuroscience, 1892-1904. |
| 25524173 | not listed | Serrano, F., et al. 2014. Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice. Molecular neurodegeneration, 61. |
| 25352748 | 1:400 | Belem de Andrade, G., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. Molecular vision, 1422-1433. |
| 24795561 | 1:1000 | Lugo, J., et al. 2014. Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins. Frontiers in molecular neuroscience, 27. |
| 23719805 | 1:1000 | Hoy, J., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. The Journal of neuroscience : the official journal of the Society for Neuroscience, 9364-9384. |
| 22561452 | 1:2 (supe) | Hu, J.H., et al. 2012. Preso1 dynamically regulates group I metabotropic glutamate receptors. Nature neuroscience, 836-844. |
| 22338020 | not listed | Qian, H., et al. 2012. β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of neurophysiology, 2703-2712. |
| 22153079 | not listed | Pavlopoulos, E., et al. 2011. Neuralized1 activates CPEB3: a function for nonproteolytic ubiquitin in synaptic plasticity and memory storage. Cell reports, 1369-1383. |
| 20573181 | 1:1000 | Pham, E., et al. 2010. Progressive accumulation of amyloid-beta oligomers in Alzheimer's disease and in amyloid precursor protein transgenic mice is accompanied by selective alterations in synaptic scaffold proteins. The FEBS journal, 3051-3067. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 31633032 | 1:500 | Werner, C.T., et al. 2019. Ubiquitin-proteasomal regulation of chromatin remodeler INO80 in the nucleus accumbens mediates persistent cocaine craving. Science advances, eaay0351. |
| 30143647 | 1:1000 | Lee, S.H., et al. 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature Communications, 3434. |
| 29735556 | 1:1000 | Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5289-5301. |
| 29429936 | not listed | Matt, L., et al. 2018. α-Actinin Anchors PSD-95 at Postsynaptic Sites. Neuron, 1094-1109. |
| 29284046 | 1:1000 | Dosemeci, A., et al. 2017. IRSp53 accumulates at the postsynaptic density under excitatory conditions. PLoS one, e0190250. |
| 27307232 | not listed | Yokoi ,N., et al. 2016. Identification of PSD-95 Depalmitoylating Enzymes. The Journal of neuroscience : the official journal of the Society for Neuroscience, 6431-6444. |
| 26547831 | 1:5000 | MacGillavry, H., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. The European journal of neuroscience, 179-193. |
| 26328538 | 1:20 (supe) | Pinheiro, S., et al. 2016. Tau Mislocation in Glucocorticoid-Triggered Hippocampal Pathology. Molecular neurobiology, 4745-4753. |
| 26679993 | 1:200 | Stanic, J., et al. 2015. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nature Communications, 10181. |
| 25526735 | not listed | Gill, I., et al. 2015. Presynaptic NMDA receptors - dynamics and distribution in developing axons in vitro and in vivo. Journal of cell science, 768-780. |
| 24530450 | not listed | Tao-Cheng, J.H., et al. 2014. Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation. Neuroscience, 80-90. |
| 21961035 | 1:500 | Jarome, T., et al. 2011. Activity dependent protein degradation is critical for the formation and stability of fear memory in the amygdala. PLoS one, e24349. |
| 21795692 | not listed | Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34839-34850. |
| 20347015 | 1:200, 1:1000 | Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 11-17. |
| 20220006 | not listed | Perez de Arce, K., et al. 2010. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation. The Journal of neuroscience : the official journal of the Society for Neuroscience, 3728-3738. |
| 20164337 | not listed | Zibetti, C., et al. 2010. Alternative splicing of the histone demethylase LSD1/KDM1 contributes to the modulation of neurite morphogenesis in the mammalian nervous system. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2521-2532. |