Anti-Pan-FHF-A Antibody (N235/22)

Name: Anti-Pan-FHF-A Antibody
Brand: NeuroMab
SKU: 75-246
Price: 319.0 USD
Availability: InStock

Our Anti-Pan-FHF-A mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N235/22. It detects human, mouse, and rat Pan-FHF-A, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-246

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Pan-FHF-A

Target Description

FGF12A/FHF1A and FGF14A/FHF4A are members of the fibroblast growth factor homologous factors family (a subfamily of fibroblast growth factors.). FHFs have a role in regulation of voltage gated sodium channels (Nav channels) and are thought to be involved in nervous system development.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N235/22

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

FGF13 FHF2

Molecular Weight

30 kDa

Antigen

Synthetic peptide amino acids 2-18 (AAAIASSLIRQKRQARE) of human FHF2A (accession number Q92913)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10697884

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with FGF12A/FHF1A and FGF14A/FHF4ADoes not cross-react with FGF13B/FHF2B

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

cross reacts with FGF12A/FHF1A and FGF14A/FHF4A

UniProt Details

UniProt (Human): Q92913
UniProt (Immunogen Species): Q92913

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
27999940	1:500	Barbosa, C., et al. 2017. FHF2 isoforms differentially regulate Nav1.6-mediated resurgent sodium currents in dorsal root ganglion neurons.. Pflugers Archiv, 195-212.

Western Blot: Mouse
PMID	Dilution	Publication
37516919	1:300	Lesage, A., et al. 2023. Determinants of iFGF13-mediated regulation of myocardial voltage-gated sodium (NaV) channels in mouse. The Journal of general physiology, 0.
27666389	0.5ug/ml	Dover, K., et al. 2016. FHF-independent conduction of action potentials along the leak-resistant cerebellar granule cell axon. Nature Communications, 12895.
26889602	1:1000	Bosch, M.K., et al. 2016. Proteomic analysis of native cerebellar iFGF14 complexes.. Channels, 297-312.
25429153	not listed	Venkatesan, K., et al. 2014. Fast-onset long-term open-state block of sodium channels by A-type FHFs mediates classical spike accommodation in hippocampal pyramidal neurons.. Journal of Neuroscience, 16126-16139.
20679355	not listed	Dover, K., et al. 2010. Long-term inactivation particle for voltage-gated sodium channels.. Journal of Physiology, 3695-3711.

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Anti-Pan-FHF-A Antibody (N235/22)

SKU: 75-246

Product Details

Product Specific References for Applications and Species

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