Anti-NGL-2/LRRC4 Antibody (N50/36)

Name: Anti-NGL-2/LRRC4 Antibody
Brand: NeuroMab
SKU: 75-084
Price: 329.0 USD
Availability: InStock

Our Anti-NGL-2/LRRC4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N50/36. It is KO validated, detects mouse and rat NGL-2/LRRC4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N50/36 (left) or K89/41 (right) TC supe.

Adult mouse brain immunoblot: extracts of brains from wild-type (1), Netrin-G2 knockout (2) and NGL-2 knockout (3) mice were probed with Netrin-G2 antibody (left) or N50/36 (right). Reproduced with permission from 2007 Zhang et al Genes Brain Behav.

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Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-084

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NGL-2/LRRC4

Scientific Background

Leucine Rich Repeat Containing 4 is encoded by the gene LRRC4. LRRC4 is a synaptic adhesion protein that regulates the formation of excitatory synapses through the recruitment of pre and postsynaptic proteins. LRRC4 organizes the lamina pathway-specific differentiation of dendrites, and plays an important role in auditory synaptic responses. LRRC4 is expressed in the brain. LRRC4 is significantly downregulated in primary brain tumors.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N50/36

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Lrrc4

Molecular Weight

100 kDa

Immunogen

Fusion protein amino acids 550-652 of mouse NGL-2 (accession number AAG60620) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2137614

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.14

Dilution Ranges

IHC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against NGL-1 or NGL-3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat-containing protein 4 (Brain tumor-associated protein MBAG1) (Netrin-G2 ligand) (NGL-2)

UniProt ID

Q99PH1

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
29553369	1:100	Soto, F., et al. 2018. Synapse maintenance and restoration in the retina by NGL2. eLife, e30388.
23864682	1:100	Soto, F., et al. 2013. NGL-2 regulates pathway-specific neurite growth and lamination, synapse formation, and signal transmission in the retina. The Journal of neuroscience : the official journal of the Society for Neuroscience, 11949-11959.

Western Blot: Mouse
PMID	Dilution	Publication
23177961	not listed	DeNardo, L., et al. 2012. NGL-2 regulates input-specific synapse development in CA1 pyramidal neurons. Neuron, 762-775.
17973922	not listed	Zhang, W., et al. 2008. Netrin-G2 and netrin-G2 ligand are both required for normal auditory responsiveness. Genes, brain, and behavior, 385-392.

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Anti-NGL-2/LRRC4 Antibody (N50/36)

SKU: 75-084

Product Details

Product Specific References for Applications and Species

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